Post-genomic Pseudomonas 英文参考文献.docVIP

/2001/3/1/reports/4002.1 Meeting report Post-genomicPseudomonas Shawn Lewenza and Robert EW Hancock Address: Department of Microbiology and Immunology, 6174 University Boulevard, University of British Columbia, Vancouver, V6T 1Z3, Canada. Correspondence: Robert EW Hancock. E-mail: bob@cmdr.ubc.ca Published: 7 December 2001 GenomeBiology 2001, 3(1):reports4002.1–4002.2 The electronic version of this article is the complete one and can be found online at /2001/3/1/reports/4002 ? BioMed Central Ltd (Print ISSN 1465-6906; Online ISSN 1465-6914) from the lab of Frederick Ausubel (Harvard Medical School, A report on the Pseudomonas 2001 Meeting, Brussels, Belgium, 17-21 September 2001. Boston, USA) and a talk from Laurence Rahme (at the same school) described approaches to identifying P. aeruginosa genes that are necessary for pathogenesis in a number of hosts. Transposon mutants were constructed and tested for The genus Pseudomonas is renowned for its ecological diver- sity, its pathogenic potential in a range of hosts (insects, nematodes, plants and humans) and its potential for use in degrading environmental contaminants (bioremediation) and preventing infections of important food crops (biocon- trol). With the release of the Pseudomonas aeruginosa genome one year ago and the near-completion of the genome sequences of Pseudomonas putida (reported on at this meeting by Burkhard Tümmler, Medizinische Hochschule virulence in high-throughput insect, plant and nematode models of infection. The Ausubel lab intends to identify each transposon insertion site and ultimately produce a non- redundant library of approximately 3,000-5,000 P. aerugi- nosa mutants that will be made publicly available. Signature-tagged mutagenesis (STM) is an alternative muta- genesis method that is based on the negative selection of unique oligonucleotide-tagged transposon mut