Preferential Genome Targeting of the CBP Co-Activator by Rel and Smad Proteins in Early Drosophila melanogaster Embryos 英文参考文献.docVIP

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Preferential Genome Targeting of the CBP Co-Activator by Rel and Smad Proteins in Early Drosophila melanogaster Embryos 英文参考文献.doc

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Preferential Genome Targeting of the CBP Co-Activator by Rel and Smad Proteins in Early Drosophila melanogaster Embryos 英文参考文献

PreferentialGenomeTargetingoftheCBPCo-Activator byRelandSmadProteinsinEarlyDrosophila melanogasterEmbryos Per-HenrikHolmqvist1,AnnBoija1,PhilgePhilip2,3,FilipCrona1,PerStenberg2,3*,MattiasMannervik1* 1TheWenner-GrenInstitute,DevelopmentalBiology,StockholmUniversity,Stockholm,Sweden,2DepartmentofMolecularBiology,Umea? University,Umea?, Sweden, 3ComputationalLifeScienceCluster(CLiC),Umea? University,Umea?, Sweden Abstract CBP and the related p300 protein are widely used transcriptional co-activators in metazoans that interact with multiple transcriptionfactors.WhetherCBP/p300occupiesthegenomeequallywithallfactorsorpreferentiallybindstogetherwith some factors is not known. We therefore compared Drosophila melanogaster CBP (nejire) ChIP–seq peaks with regions boundby40differenttranscriptionfactorsinearlyembryos,andwefoundhighco-occupancywiththeRel-familyprotein Dorsal.DorsalisrequiredforCBPoccupancyintheembryo,butonlyatregionswherefewotherfactorsarepresent.CBP peaksinmutantembryoslackingnuclearDorsalarebestcorrelatedwithTGF-?/Dpp-signalingandSmad-proteinbinding. Differences in CBP occupancy in mutant embryos reflect gene expression changes genome-wide, but CBP also occupies some non-expressed genes. The presence of CBP at silent genes does not result in histone acetylation. We find that Polycomb-repressedH3K27me3chromatindoesnotprecludeCBPbinding,butrestrictshistoneacetylationatCBP-bound genomicsites.We conclude thatCBP occupancy inDrosophila embryospreferentially overlapsfactors controllingdorso- ventralpatterningandthatCBPbindssilentgeneswithoutcausinghistonehyperacetylation. Citation:HolmqvistP-H,BoijaA,PhilipP,CronaF,StenbergP,etal.(2012)PreferentialGenomeTargetingoftheCBPCo-ActivatorbyRelandSmadProteinsin EarlyDrosophilamelanogasterEmbryos.PLoSGenet8(6):e1002769.doi:10.1371/journal.pgen.1002769 Editor:NorbertPerrimon,HarvardMedicalSchool,HowardHughesMedicalInstitute,UnitedStatesofAmerica ReceivedFebruary9,2012;AcceptedMay2,2012;PublishedJune21,2012 Co