Preferential Transfer of Certain Plasma Membrane Proteins onto T and B Cells by Trogocytosis 英文参考文献.docVIP

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Preferential Transfer of Certain Plasma Membrane Proteins onto T and B Cells by Trogocytosis 英文参考文献.doc

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Preferential Transfer of Certain Plasma Membrane Proteins onto T and B Cells by Trogocytosis 英文参考文献

PreferentialTransferofCertainPlasmaMembrane ProteinsontoTandBCellsbyTrogocytosis SandrineDaubeuf1,2.,AnneAucher1,2.,ChristineBordier1,2,AudreySalles1,2,3,LaurentSerre1,2,Ge′rald Gaibelet1,2,4,Jean-CharlesFaye5,GillesFavre5,EtienneJoly1,2*,DenisHudrisier1,2 * 1CNRS, IPBS (Institut dePharmacologieet deBiologie Structurale),Toulouse, France, 2Universite′ deToulouse,UPS, IPBS, Toulouse, France, 3Centred’Immunologie, MarseilleLuminy,France,4INSERMU563,Ho?pitalPurpan,BP3048,Toulouse,France,5INSERMU563,InstitutClaudiusRe′gaud, Toulouse,France Abstract TandBcellscaptureantigensviamembranefragmentsofantigenpresentingcells(APC)inaprocesstermedtrogocytosis. Whether(andhow)apreferentialtransferofsomeAPCcomponentsoccursduringtrogocytosisisstilllargelyunknown.We analyzed the transfer onto murine T and B cells of a large panel of fluorescent proteins with different intra-cellular localizations in the APC or various types of anchors in the plasma membrane (PM). Only the latter were transferred by trogocytosis,albeitwithdifferentefficiencies.Unexpectedly,proteinsanchoredtothePM’scytoplasmicface,orrecruitedto it via interaction with phosphinositides, were more efficiently transferred than those facing the outside of the cell. For proteinsspanningthePM’swholewidth,transferefficiencywasfoundtovaryquitesubstantially,withtetraspanins,CD4 andFcRcfoundamongthemostefficientlytransferredproteins.Weexploitedourfindingstosetimmunodiagnosticassays basedonthecaptureofpreferentiallytransferredcomponentsontoTorBcells.Thepreferentialtransferdocumentedhere shouldproveusefulindecipheringthecellularstructuresinvolvedintrogocytosis. Citation: Daubeuf S, Aucher A, Bordier C, Salles A, Serre L, et al. (2010) Preferential Transfer of Certain Plasma Membrane Proteins onto T and B Cells by Trogocytosis.PLoSONE5(1):e8716.doi:10.1371/journal.pone.0008716 Editor:SteveH.Caplan,UniversityofNebraskaMedicalCenter,UnitedStatesofAmerica ReceivedNovember4,2009;AcceptedDecember21,2009;PublishedJanuary14,2