Protein Arrays for Multidrug-resistance in Human Leukemia Cell Determination 英文参考文献.docVIP

Sensors 2005, 5, 250-257 sensors ISSN 1424-8220 ? 2005 by MDPI /sensors Protein Arrays for Multidrug-resistance in Human Leukemia Cell Determination Juan Du 1, Baoan Chen 1,*, Chunxiu Zhang 2, Xiaoxing Xu 3, Jian Cheng 1, Feng Gao 1and Zuhong Lu 2 1 Department of Hematology, Institute of MDS, Zhongda Hospital, Southeast University Medical College, 87 Dingjia Bridge Hunan Road, Nanjing, 210009, P. R. China 2 National Laboratory for Molecular and Biomolecular Electronics (Jian-Xiong Wu Laboratory), Southeast University, Nanjing, 210096, P. R. China 3 Department of Chemistry, Changshu College of Jiangsu, Changshu, 215500, P. R. China * Corresponding author. E-mail: bachen@; Fax: 86-25 Tel: 86-25Received: 21 May 2004 / Accepted: 11 November 2004 / Published: 3 May 2005 Abstract: A novel technique was developed, that was high throughput simultaneous screening of multiple resistance protein expression based on a protein array system. The method combined the advantage of the specificity of enzyme-linked immunosorbent assays with the sensitivity and high throughput of microspot. In this system, the multiple resistance protein arrays were created by spotting the captured antibodies onto the glass slide. The arrays were then incubated with cell samples of leukemia patients. The bound proteins were recognized by biotin-conjugated antibodies and detected by CCD. Experiments demonstrated that three multiple resistance proteins, including Pgp, MRP and BCRP which are members of the ATP-binding-cassette (ABC) superfamily of membrane transporters could be simultaneously detected using this new approach. Research work shows the result is coincident with flow cytometry (FCM) (P0.01). It provided a methodology to develop many high-density protein array systems to detect a variety of proteins. The protein arrays will provide a powerful tool to ident