Protein Domain of Unknown Function 3233 is a Translocation Domain of Autotransporter Secretory Mechanism in Gamma proteobacteria 英文参考文献.docVIP

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Protein Domain of Unknown Function 3233 is a Translocation Domain of Autotransporter Secretory Mechanism in Gamma proteobacteria 英文参考文献.doc

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Protein Domain of Unknown Function 3233 is a Translocation Domain of Autotransporter Secretory Mechanism in Gamma proteobacteria 英文参考文献

ProteinDomainofUnknownFunction3233isa TranslocationDomainofAutotransporterSecretory MechanisminGammaproteobacteria AnanthPrakash1,YogeeshwariS.1,SanchariSircar1,ShipraAgrawal1,2* 1InstituteofBioinformaticsandAppliedBiotechnology,Bangalore,India,2BioCOSLifeSciencesPrivateLimited,Bangalore,India Abstract Vibriocholerae,theenteropathogenicgramnegativebacteriaisoneofthemaincausativeagentsofwaterbornediseases likecholera.About1/3rd oftheorganism’sgenomeisuncharacterisedwithmanyproteincodinggeneslackingstructure andfunctional information. These proteins form significant fractionof the genome andarecrucial in understanding the organism’s complete functional makeup. In this study we report the general structure and function of a family of hypothetical proteins, Domain of Unknown Function 3233 (DUF3233), which are conserved across gram negative gammaproteobacteria(especiallyinVibriosp.andsimilarbacteria).ProfileandHMMbasedsequencesearchmethodswere used to screen homologues of DUF3233. The I-TASSER fold recognition method was used to build a three dimensional structuralmodelofthedomain.Thestructureresemblesthetransmembranebeta-barrelwithanaxialN-terminalhelixand twelveantiparallelbeta-strands.Usingacombinationofamphipathyanddiscriminationanalysisweanalysedthepotential transmembrane beta-barrel forming properties of DUF3233. Sequence, structure and phylogenetic analysis of DUF3233 indicates that this gram negative bacterial hypothetical protein resembles the beta-barrel translocation unit of autotransporterVasecretorymechanismwithageneorganisationthatdiffersfromtheconventionalVasystem. Citation:PrakashA,YogeeshwariS,SircarS,AgrawalS(2011)ProteinDomainofUnknownFunction3233isaTranslocationDomainofAutotransporterSecretory MechanisminGammaproteobacteria.PLoSONE6(11):e25570.doi:10.1371/journal.pone.0025570 Editor:EugeneA.Permyakov,RussianAcademyofSciences,InstituteforBiologicalInstrumentation,RussianFederation ReceivedAugust15,2011;AcceptedSeptember7,2011;PublishedNovember1,20