Proteomics gets faster and smarter 英文参考文献.docVIP

Meeting report Proteomics gets faster and smarter Hannah Johnson and Simon J Gaskell Address: Michael Barber Centre for Mass Spectrometry, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK. Correspondence: Simon J Gaskell. Email: Simon.Gaskell@manchester.ac.uk Published: 15 November 2006 Genome Biology 2006, 7:331 (doi:10.1186/gb-2006-7-11-331) The electronic version of this article is the complete one and can be found online at /2006/7/11/331 ? 2006 BioMed Central Ltd The importance of quantification A report on the third annual joint meeting of the British To help understand how an organism works as a whole, it is important to quantify proteins accurately following their identification. Rob Beynon (University of Liverpool, UK) stressed the need for both relative and absolute quantitative proteomics. He reported a novel methodology (QconCAT [/index.php?id=qcats/index]) for absolute quantification of proteins that is able to simultaneously quantify a number of different proteins Society for Proteome Research and the European Bioinformatics Institute, Hinxton, UK, 12-14 July 2006. A recent meeting at the European Bioinformatics Institute outside Cambridge, UK, focused on the need to integrate current proteomics methods with advances in both mass. spectrometric (MS) technology and bioinformatics software. The increasing sensitivity of MS coupled with enhanced separation techniques mean that the quantity and quality of within a complex proteome. QconCATs are synthetic proteins that are designed to encode internal standards for a number of different proteins. The protein is expressed the data produced from high-throughput studies are (typically in heavy-isotope-labeled form), purified, increasing continuously. Without bioinformatics techniques to analyze these data comprehensively, the