Purification and Identification of Antioxidant Peptides from Enzymatic Hydrolysates of Tilapia (Oreochromis niloticus) Frame Protein 英文参考文献.docVIP
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Purification and Identification of Antioxidant Peptides from Enzymatic Hydrolysates of Tilapia (Oreochromis niloticus) Frame Protein 英文参考文献
Molecules 2012, 17, 12836-12850; doi:10.3390/molecules171112836
OPEN ACCESS
molecules
ISSN 1420-3049
/journal/molecules
Article
Purification and Identification of Antioxidant Peptides from
Enzymatic Hydrolysates of Tilapia (Oreochromis niloticus)
Frame Protein
Jian Fan, Jintang He, Yongliang Zhuang and Liping Sun *
College of Chemistry and Engineering, Kunming University of Science and Technology,
Kunming 650500, China; E-Mails: fj_3332000@ (J.F.); kmustslp@ (J.H.);
kmylzhuang@ (Y.Z.)
* Author to whom correspondence should be addressed; E-Mail: kmlpsun@;
Tel.: +86-871-5920-302; Fax: +86-871-3801-114.
Received: 29 September 2012; in revised form: 26 October 2012 / Accepted: 29 October 2012 /
Published: 1 November 2012
Abstract: Tilapia frame protein was hydrolyzed by different proteases, including properase E,
pepsin, trypsin, flavourzyme, neutrase, gc106 and papain, to obtain antioxidant peptides.
The tilapia frame protein hydrolysate (TFPH) obtained by trypsin exhibited the highest
degree of hydrolysis and antioxidant activity. Three series of peptides (TFPH1, TFPH 2
and TFPH 3) were obtained by ultrafiltration of TFPH through molecular weight
cut-off membranes of 5, 3 and 1 kDa, respectively, and their IC50 values on scavenging
1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide anion radical (?O2), hydrogen
peroxides (H2O2) and hydroxyl radical (?OH) activities were determined and compared
with glutathione (GSH). The results showed that TFPH1 had the highest antioxidant
activity. TFPH1 was further purified using ion exchange chromatography, gel filtration
chromatography, and reversed phase high performance liquid chromatography (RP-HPLC).
Finally, two antioxidant peptides were identified and the amino acid sequences were
identified as Asp-Cys-Gly-Tyr (456.12 Da) and Asn-Tyr-Asp-Glu-Tyr (702.26 Da),
respectively. The IC50 values of two peptides on hydro
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