Puerarin Suppresses Invasion and Vascularization of Endometriosis Tissue Stimulated by 17β-Estradiol 英文参考文献.docVIP

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Puerarin Suppresses Invasion and Vascularization of Endometriosis Tissue Stimulated by 17β-Estradiol 英文参考文献.doc

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Puerarin Suppresses Invasion and Vascularization of Endometriosis Tissue Stimulated by 17β-Estradiol 英文参考文献

PuerarinSuppressesInvasionandVascularizationof EndometriosisTissueStimulatedby17b-Estradiol DanWang3.,YuhuanLiu3.,JieHan1,DongxiaZai1,MeiJi3,WenCheng1,LingXu1,LuxiYang2 ,Miaoxia He4,JianNi5,ZailongCai2*,ChaoqinYu1* 1Department of Chinese Traditional Medicine, Changhai Hospital, Second Military Medical University, Shanghai, China, 2Clinical Research Center, Changhai Hospital, SecondMilitaryMedicalUniversity,Shanghai,China,3DepartmentofObstetricsandGynecology,ChanghaiHospital,SecondMilitaryMedicalUniversity,Shanghai,China, 4DepartmentofPathology,ChanghaiHospital,SecondMilitaryMedicalUniversity,Shanghai,China,5InstituteofMicro/NanoScienceandTechnology,ShanghaiJiaotong University,Shanghai,China Abstract Background:Puerarin,aphytoestrogenwithaweakestrogeniceffect,bindstoestrogenreceptors,therebycompetingwith 17b-estradiol(E2)andproducingananti-estrogeniceffect.Thisstudywastoinvestigatewhetherpuerarincouldsuppress theinvasionandvascularizationofE2-stimulatedendometriotictissue. Methodology/Principal Findings:Theendometrioticstromalcells(ESCs)weresuccessfullyestablished andtheirinvasive ability under different treatments was assessed through a Transwell Assay. Simultaneously, matrix metallopeptidase 9 (MMP-9) and tissue inhibitor of metalloproteinase 1 (TIMP-1) were detected by western blotting. Vascularization of endometriotictissueswasobservedbychickenchorioallantoicmembrane(CAM)assay.ThestainingofMMP-9,intercellular adhesionmolecule1(ICAM-1),TIMP-1,andvascularendothelialgrowthfactor(VEGF)ingraftedendometriotictissueswas examined using immunohistochemistry analysis. The purity of ESCs in isolated cells was .95%, as determined by the fluoroimmunoassayofvimentin.E2(1028mol/L)promotedtheinvasivenessofESCsbyincreasingMMP-9accumulationand decreasingTIMP-1accumulation.Interestingly,puerarin(1029mol/L)significantlyreversedtheseeffects(P,0.01).TheCAM assay indicated that puerarin (1029mol/L) also inhibited the angiopoiesis of endometriotic tissue stimulated by the E2 (1028