QTLs and candidate genes for desiccation and abscisic acid content in maize kernels 英文参考文献.docVIP
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QTLs and candidate genes for desiccation and abscisic acid content in maize kernels 英文参考文献
Capelleetal.BMCPlantBiology2010,10:2
/1471-2229/10/2
RESEARCH ARTICLE
OpenAccess
QTLsandcandidategenesfordesiccationand
abscisicacidcontentinmaizekernels
ValérieCapelle1,2,CarineRemoué2,3,LaurenceMoreau4,AgnèsReyss1,2,AlineMahé1,2,AgnèsMassonneau5,6,
MatthieuFalque4,AlainCharcosset4,ClaudineThévenot1,2,PeterRogowsky5,SylvieCoursol4,Jean-LouisPrioul1,2*
Abstract
Background:Kernelmoistureatharvestisanimportanttraitsincealowvalueisrequiredtopreventunexpected
earlygerminationandensureseedpreservation.Itisalsowellknownthatearlygerminationoccursinviviparous
mutants,whichareimpairedinabscisicacid(ABA)biosynthesis.Toprovidesomeinsightintothegenetic
determinismofkerneldesiccationinmaize,quantitativetraitloci(QTLs)weredetectedfortraitsrelatedtokernel
moistureandABAcontentinbothembryoandendospermduringkerneldesiccation.Inparallel,theexpression
andmappingofgenesinvolvedinkerneldesiccationandABAbiosynthesis,wereexaminedtodetectcandidate
genes.
Results:TheuseofanintermatedrecombinantinbredlinepopulationallowedforpreciseQTLmapping.For29
traitsexaminedinanunreplicatedtimecoursetrialofdaysafterpollination,atotalof78QTLsweredetected,43
beingrelatedtokerneldesiccation,15tokernelweightand20toABAcontent.MultiQTLmodelsexplained35to
50%ofthephenotypicvariationfortraitsrelatedtowaterstatus,indicatingalargegeneticcontrolamenableto
breeding.Tenofthe20locicontrollingABAcontentcolocatedwithpreviouslydetectedQTLscontrollingwater
statusandABAcontentinwaterstressedleaves.Mappingofcandidategenesassociatedwithkerneldesiccation
andABAbiosynthesisrevealedseveralcolocationsbetweengeneswithputativefunctionsandQTLs.Parallel
investigationviaRT-PCRexperimentsshowedthattheexpressionpatternsoftheABA-responsiveRab17andRab28
genesaswellasthelateembryogenesisabundantEmb5andaquaporingeneswererelatedtodesiccationrate
andparentalalleleeffect.Databasesearchesledtotheidentificationandmappingoftwozeaxanthinepoxidase
(ZEP)andfivenovel9-cis-epoxycarotenoiddioxygenase(NCED)relatedgenes,bothgenefamiliesbeinginvolvedi
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