Quantitative Proteomic Analysis of the Effect of Fluoride on the Acquired Enamel Pellicle 英文参考文献.docVIP

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Quantitative Proteomic Analysis of the Effect of Fluoride on the Acquired Enamel Pellicle 英文参考文献.doc

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Quantitative Proteomic Analysis of the Effect of Fluoride on the Acquired Enamel Pellicle 英文参考文献

QuantitativeProteomicAnalysisoftheEffectofFluoride ontheAcquiredEnamelPellicle WalterL.Siqueira1*,MeltemBakkal1,2,YizhiXiao1,JenniferN.Sutton3,FaustoM.Mendes4 1DepartmentofBiochemistryandSchoolofDentistry,SchulichSchoolofMedicineandDentistry,WesternUniversity,London,Onatrio,Canada,2DepartmentofPediatric Dentistry,School of Dentistry, Marmara University, Istanbul, Turkey, 3Thermo Fisher Scientific, Cambridge, Massachusetts, United States of America, 4Department of PediatricDentistry,FacultyofDentistry,UniversityofSa?o Paulo,Sa?o Paulo,Sa?o Paulo,Brazil Abstract Theacquiredenamelpellicle(AEP)isathinfilmformedbytheselectiveadsorptionofsalivaryproteinsontotheenamel surface of teeth. The AEP forms a critical interface between the mineral phase of teeth (hydroxyapatite) and the oral microbial biofilm. This biofilm is the key feature responsible for the development of dental caries. Fluoride on enamel surfaceiswellknowntoreducecariesbyreducingthesolubilityofenameltoacid.Informationontheeffectsoffluorideon AEP formation is limited. This study aimed to investigate the effects of fluoride treatment on hydroxyapatite on the subsequent formation of AEP. In addition, this study pioneered the use of label-free quantitative proteomics to better understandthecompositionofAEPproteins.Hydroxyapatitediscswererandomlydividedin4groups(n=10pergroup). Each disc was exposed to distilled water (control) or sodium fluoride solution (1, 2 or 5%) for 2hours. Discs were then washedandimmersedinhumansalivaforanadditional2hours.AEPfromeachdiscwascollectedandsubjectedtoliquid chromatographyelectrosprayionizationmassspectrometryforproteinidentification,characterizationandquantification.A totalof45proteinswerepresentinallfourgroups,12proteinswereexclusivelypresentinthecontrolgroupandanother19 proteinswereonlypresentinthediscstreatedwith5%sodiumfluoride.Relativeproteomicquantificationwascarriedout forthe45proteinsobservedinallfourgroups.Notably,theconcentrationofimportantsalivaryproteins,suchas