Rapid Visual Tests Fast and Reliable Detection of Ochratoxin A 英文参考文献.docVIP

Toxins 2010, 2, 2230-2241; doi:10.3390/toxins2092230 OPEN ACCESS toxins ISSN 2072-6651 /journal/toxins Review Rapid Visual Tests: Fast and Reliable Detection of Ochratoxin A Ingrid Bazin *, Elodie Nabais and Miguel Lopez-Ferber Ecole des Mines d’Ales, 6 av. de Clavieres 30319, Ales cedex, France; E-Mails: elodie.nabais@mines-ales.fr (E.N.); Miguel.Lopez-Ferber@mines-ales.fr (M.L.-F.) * Author to whom correspondence should be addressed; E-Mail: ingrid.bazin@mines-ales.fr; Tel.: +33-466-782-724; Fax: +33-466-782-701. Received: 2 August 2010; in revised form: 13 August 2010 / Accepted: 23 August 2010 / Published: 26 August 2010 Abstract: This paper reviews the early detection strategies that have been employed for the rapid monitoring of ochratoxin A (OTA) contamination of food. OTA, a mycotoxin mainly produced by some Aspergillus and Penicillium species, is found in cereals, coffee, wine, pork and grapes. To minimize the entry of this mycotoxin into the food chain, rapid diagnostic tools are required. To this end, the potential use of lateral flow devices has also been developed. In this study, we analyze the robustness of test strips using published methods for colorimetric detection. Different test formats are discussed, and challenges in the development of lateral flow devices for on-site determination of OTA, with requirements such as robustness, speed, and cost-effectiveness, are discussed. Keywords: ochratoxin A; colorimetric test; lateral flow test; flow-through test; clean-up immunoassay 1. Introduction Rapid diagnostic assays were originally developed in the medical and clinical sectors; the urine glucose and pregnancy test strips were the first to be commercialized. The success of these tests, due to their speed and ease of use, led to their implementation in other fields, such as in the animal health and foodstuff industries. Since then, many laboratories have developed rapid analysis s