Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules 英文参考文献.docVIP

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Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules 英文参考文献.doc

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Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules 英文参考文献

Sensors 2010, 10, 5927-5948; doi:10.3390/s100605927 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Review Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules Gamal Rayan *, Jean-Erik Guet, Nicolas Taulier, Frederic Pincet and Wladimir Urbach Laboratoire de Physique Statistique de l’Ecole Normale Supérieure, associe aux Universites Paris 6 et Paris 7, CNRS UMR 8550, 24 rue Lhomond, 75005 Paris, France; E-Mails: jean-erik.guet@lps.ens.fr (J-E.G.); nicolas.taulier@upmc.fr (N.T.); pincet@lps.ens.fr (F.P.); urbach@lps.ens.fr (W.U.) * Author to whom correspondence should be addressed; E-Mail: gamal.rayan@lps.ens.fr; Tel.: +33-0-1-44-32-34-48; Fax: +33-0-1-44-32-34-33. Received: 20 April 2010; in revised form: 10 May 2010 / Accepted: 28 May 2010 / Published: 10 June 2010 Abstract: This review examines some recent applications of fluorescence recovery after photobleaching (FRAP) to biopolymers, while mainly focusing on membrane protein studies. Initially, we discuss the lateral diffusion of membrane proteins, as measured by FRAP. Then, we talk about the use of FRAP to probe interactions between membrane proteins by obtaining fundamental information such as geometry and stoichiometry of the interacting complex. Afterwards, we discuss some applications of FRAP at the cellular level as well as the level of organisms. We conclude by comparing diffusion coefficients obtained by FRAP and several other alternative methods. Keywords: biomembranes; Fluorescence Recovery after Photobleaching (FRAP); diffusion; biomolecular interactions Abbreviations: FRAP—fluorescence recovery after photobleaching FRAPP—fluorescence recovery after fringe-pattern photobleaching FCS—fluorescence correlation spectroscopy SPT—single particle tracking 5928 Sensors 2010, 10 D—diffusion coefficient R—radius SD—Saffman Delbrück GUV—giant unilamellar vesicle FFEM—freeze fracture electr