Rickettsia Species in African Anopheles Mosquitoes 英文参考文献.docVIP

RickettsiaSpeciesinAfricanAnophelesMosquitoes CristinaSocolovschi1,Fre′de′ricPages2,MamadouO.Ndiath3,PavelRatmanov1,DidierRaoult1* 1Aix Marseille Universite′, URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, Marseille, France, 2CIRE/ARS Oce′an Indien, La Re′union, France, 3IRD, Laboratoire de Paludologie,UMR198URMITE,Dakar,Se′ne′gal Abstract Background:ThereishigherrateofR.felisinfectionamongfebrilepatientsthaninhealthypeopleinSub-SaharanAfrica, predominantly in the rainy season. Mosquitoes possess a high vectorial capacity and, because of their abundance and aggressiveness,likelyplayaroleinrickettsialepidemiology. Methodology/PrincipalFindings:QuantitativeandtraditionalPCRassaysspecificforRickettsiagenesdetectedrickettsial DNAin13of848(1.5%)AnophelesmosquitoescollectedfromCo?ted’Ivoire,Gabon,andSenegal.R.feliswasdetectedinone An. gambiae molecular form S mosquito collected from Kahin, Co?te d’Ivoire (1/77, 1.3%). Additionally, a new Rickettsia genotypewasdetectedinfiveAn.gambiaemolecularformSmosquitoescollectedfromCo?ted’Ivoire(5/77,6.5%)andone mosquito from Libreville, Gabon (1/88, 1.1%), as well as six An. melas (6/67, 9%) mosquitoes collected from Port Gentil, Gabon.AsequenceanalysisofthegltA,ompB,ompAandsca4genesindicatedthatthisnewRickettsiasp.iscloselyrelated toR.felis.NorickettsialDNAwasdetectedfromAn.funestus,An.arabiensis,orAn.gambiaemolecularformMmosquitoes. Additionally,aBLASTanalysisofthegltAsequencefromthenewRickettsiasp.resultedina99.71%sequencesimilarityto aspecies(JQ674485)previouslydetectedinabloodsampleofaSenegalesepatientwithafeverfromtheBandafassivillage, Kedougouregion. Conclusion: R. felis was detected for the first time in An. gambiae molecular form S, which represents the major African malariavector.ThediscoveryofR.felis,aswellasanewRickettsiaspecies,inmosquitoesraisesnewissueswithrespectto Africanrickettsialepidemiologythatneedtobeinvestigated,suchasbacterialisolation,thedegreeofthevectorialcapacity ofmosquitoes,theanimalreservoirs,andhuma