Role of Cellular Lipids in Positive-Sense RNA Virus Replication Complex Assembly and Function 英文参考文献.docVIP

Viruses 2010, 2, 1055-1068; doi:10.3390/v2051055 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Review Role of Cellular Lipids in Positive-Sense RNA Virus Replication Complex Assembly and Function Kenneth A. Stapleford 1 and David J. Miller 2,3,* 1 Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06519, USA; E-Mail: kenneth.stapleford@ 2 Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, USA 3 Department of Microbiology Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA * Author to whom correspondence should be addressed; E-Mail: milldavi@; Tel.: +1-734-763-0565; Fax: +1-734-615-5534. Received: 31 January 2010; in revised form: 7 April 2010 / Accepted: 22 April 2010 / Published: 29 April 2010 Abstract: Positive-sense RNA viruses are responsible for frequent and often devastating diseases in humans, animals, and plants. However, the development of effective vaccines and anti-viral therapies targeted towards these pathogens has been hindered by an incomplete understanding of the molecular mechanisms involved in viral replication. One common feature of all positive-sense RNA viruses is the manipulation of host intracellular membranes for the assembly of functional viral RNA replication complexes. This review will discuss the interplay between cellular membranes and positive-sense RNA virus replication, and will focus specifically on the potential structural and functional roles for cellular lipids in this process. Keywords: RNA viruses; replication; membranes; lipids 1. Introduction The virosphere is large, complex, and continually expanding. One group of viruses responsible for a wide range of diseases in humans, animals, and plants are classified as positive-sense RNA viruses  Viruses 2010, 2 1056 due to their genome structure, which consists of one or more single-stranded RN