红蓼种子中杀黏虫活性成分的初步分析.pdfVIP

I:U影低)} 2011 , 37(4);101 -105 Plant Protection 红寥种子中杀黠虫活性成分的初步分析 姚赛群1 ， 胡冠芳2 ， 刘敏艳2 ， 张新瑞2 赞 (1.甘肃农业大学， 22州 730070; 2. 甘肃省农业科学院樵物保护研究所，二兰州 730070) 摘要 [阔的]对红寥种子乙酸乙躏萃取物中具有潜在杀St~舌性的化合物进行初步分析，为指导开发利用杀st 植物 资源提供依据。[方法]采用政脱柱层析和气相色谱-，Ji普CGC-MS)联刑法，以 5 龄私st 为试St;对红寥种子萃取物 的有效杀虫治性成分进行分析，应用色谱峰面积归一法测定各组分的相对百分含爱。[给采]极性(甲醇)组分共分 离出 37 个色谱峰，鉴定了其中的 35 个组分，占总含爱的 94.46% 。弱极性(二氯甲炕)组分共分离出 39 个色谱峰， 鉴定出 39 个组分。〔结论]从成分的化学结构推断，红寥种子的杀虫活性可能存在于 14 种成分中，.i.奖成分为制、 麟、开环格和Ji.环三榕炕类化合物。 关键诩 红与民种子p 和St; 杀st 活性; 气相色谱…质谱 中圈分类号: S 482.39 文献标识码: A 肌)1: 10. 3969/j. issn. 0529 咛 1542.2011. 04. 021 A preliminary analysis of the active ingredients from the seeds of Polygonum orientαle against Mythimna separata Yao Saiqun1 , Hu Guanfang2, Liu Minyan2 , Zhang xi盯ui2 (1. Gansu Agricultural University , Lanzhou 730070 , Chína I 2. Institute 01 Plant Protection ， 伽nsu Academy o[ Agricultural Scie时衍，Lanzhou 730070 , Chína) Abstract [Objective] Active ingredients of the ethyl acetate fraction from seeds of Polygonum orientale Linn. were analyzed to provide the basis for the exploitation of the insecticidal plant resources. [Method] Insecticidal ingredients from the seeds of P. orientale fractions were analyzed against the 5th instar larvae of Mythimna sepa- raωWalker by using silica gel column翩chromatographer (CC) and gas chromatography-mass s肝ctrometry CGC- MS). The relative percentage of each composition was determined by area normalízatíon. [Result] Thirty-seven peaks were found , 35 of which were identifíed , repre坦enting 94.46% of the total contents in polar fraction. Thir嗣 ty翩nine peaks were found , all of which were identified in low polar fraction. [Conc