Sequencing and characterization of the FVBNJ mouse genome 英文参考文献.docVIP

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Sequencing and characterization of the FVBNJ mouse genome 英文参考文献.doc

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Sequencing and characterization of the FVBNJ mouse genome 英文参考文献

Wongetal.GenomeBiology2012,13:R72 /2012/13/8/R72 RESEARCH OpenAccess SequencingandcharacterizationoftheFVB/NJ mousegenome KimWong1,SuzannahBumpstead1,LouiseVanDerWeyden1,LauraGReinholdt2,LaurensGWilming1, DavidJAdams1* andThomasMKeane1* Abstract Background:TheFVB/NJmousestrainhasitsoriginsinacolonyofoutbredSwissmiceestablishedin1935atthe NationalInstitutesofHealth.Micederivedfromthissourcewereselectivelybredforsensitivitytohistamine diphosphateandtheBstrainofFriendleukemiavirus.ThisledtotheestablishmentoftheFVB/Ninbredstrain, whichwassubsequentlyimportedtotheJacksonLaboratoryanddesignatedFVB/NJ.TheFVB/NJmousehas severaldistinctcharacteristics,suchaslargepronuclearmorphology,vigorousreproductiveperformance,and consistentlylargelittersthatmakeithighlydesirablefortransgenicstrainproductionandgeneralpurposeuse. Results:Usingnext-generationsequencingtechnology,wehavesequencedthegenomeofFVB/NJto approximately50-foldcoverage,andhavegeneratedacomprehensivecatalogofsinglenucleotidepolymorphisms, smallinsertion/deletionpolymorphisms,andstructuralvariants,relativetothereferenceC57BL/6Jgenome.We haveexaminedapreviouslyidentifiedquantitativetraitlocusforatherosclerosissusceptibilityonchromosome10 andidentifyseveralpreviouslyunknowncandidatecausalvariants. Conclusion:ThesequencingoftheFVB/NJgenomeandgenerationofthiscataloghasincreasedthenumberof knownvariantsitesinFVB/NJbyafactoroffour,andwillhelpacceleratetheidentificationoftheprecise molecularvariantsthatareresponsibleforphenotypesobservedinthiswidelyusedstrain. Background itshighreproductiveperformance,largelittersizes,and TheoriginsoftheFVB/NJlinecanbetracedbacktoan prominent pronuclei infertilized eggs that make itan outbred colony ofSwissmice(N:GP, NIHgeneral pur- idealstrainfortransgenicstrainproduction[1,3].FVB/NJ pose) established at the National Institutes of Health alsorepresentsanimportantdiseasemodel,andthisstrain (NIH)inthe1930s.Asecondcolony(N:NIH)wassubse- has been used in arange of congenic and