Serratia marcescens Is Able to Survive and Proliferate in Autophagic-Like Vacuoles inside Non-Phagocytic Cells 英文参考文献.docVIP

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Serratia marcescens Is Able to Survive and Proliferate in Autophagic-Like Vacuoles inside Non-Phagocytic Cells 英文参考文献.doc

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Serratia marcescens Is Able to Survive and Proliferate in Autophagic-Like Vacuoles inside Non-Phagocytic Cells 英文参考文献

SerratiamarcescensIsAbletoSurviveandProliferatein Autophagic-LikeVacuolesinsideNon-PhagocyticCells GriseldaV.Fedrigo1.,EmanuelM.Campoy2.,GiselaDiVenanzio1.,Mar?′aIsabelColombo2 ,Eleonora Garc?′aVe′scovi1* 1Instituto de Biolog?′a Molecular y Celular de Rosario (IBR-CONICET), Departamento de Microbiolog?′a, Facultad de Ciencias Bioqu?′micas y Farmace′uticas, Universidad Nacional de Rosario, Rosario, Argentina, 2Laboratorio de Biolog?′a Celular y Molecular-Instituto de Histolog?′a y Embriolog?′a (IHEM), Facultad de Ciencias Me′dicas, UniversidaddeCuyo,CONICET,Mendoza,Argentina Abstract Serratiamarcescensisanopportunistichumanpathogenthatrepresentsagrowingproblemforpublichealth,particularlyin hospitalizedorimmunocompromisedpatients.However,littleisknownaboutfactorsandmechanismsthatcontributetoS. marcescenspathogenesiswithinitshost.Inthiswork,weexploretheinvasionprocessofthisopportunisticpathogento epithelialcells.Wedemonstratethatonceinternalized,Serratiaisablenotonlytopersistbutalsotomultiplyinsidealarge membrane-bound compartment. This structure displays autophagic-like features, acquiring LC3 and Rab7, markers described to be recruited throughout the progression of antibacterial autophagy. The majority of the autophagic-like vacuolesinwhichSerratiaresidesandproliferatesarenon-acidicandhavenodegradativeproperties,indicatingthatthe bacteriaarecapabletoeitherdelayorpreventfusionwithlysosomalcompartments,alteringtheexpectedprogressionof autophagosomematuration.Inaddition,ourresultsdemonstratethatSerratiatriggersanon-canonicalautophagicprocess beforeinternalization.ThesefindingsrevealthatS.marcescensisabletomanipulatetheautophagictraffic,generatinga suitablenicheforsurvivalandproliferationinsidethehostcell. Citation: Fedrigo GV, Campoy EM, Di Venanzio G, Colombo MI, Ve′scovi EG (2011) Serratia marcescens Is Able to Survive and Proliferate in Autophagic-Like VacuolesinsideNon-PhagocyticCells.PLoSONE6(8):e24054.doi:10.1371/journal.pone.0024054 Editor:RobinCharlesMay,Unive