Sequential GTG-RBA banding pattern in prometaphase chromosomes of cattle (Bos taurus L) 英文参考文献.docVIP

1 Universita, degli Studi di Napoli, Dipartimento di Scienza della Produzione Animale, 80055, Portici, Naples; 2 (Received 14 November 1990; accepted 17 April 1991) A GTG-RBA banding procedure, performed for the first time on Summary - sequential the same prometaphase chromosomes of cattle, is presented with the aim of establishing to the establishment of new standard GTG and RBA-banded karyotypes at prometaphase level, useful for the precise identification of chromosomal abnormalities, comparative cytogenetics and gene mapping in the species Bos taurus L. sequential banding / GTG-bands / RBA-bands / standardization / cattle G R et technique de mise en évidence séquentielle de bandes G et R est présentée pour la première entre les bandes G et R. Les résultats de cette étude ont contribué à l’établissement de nouveaux caryotypes standards en bandes G et R au niveau prométaphasique. Cette étude est utile pour l’identification précise des anomalies chromosomiques, pour les études de cytogénétique comparée et pour la localisation des gènes de Bos taurus. bandes séquentielles / bandes G / bandes R / standardisation / bovin *  The Reading conference on the standardization of banded karyotypes of domestic animals (Ford et al, 1980), undoubtedly one of the most important steps in the history of animal cytogenetics, provided for the first time the ’standard’ G-banded karyotype of cattle (Bos taurus L) as well as that of other domestic species. Despite its excellent quality, the bovine standard G-banded karyotype soon revealed some limitations for chromosome identification, due mainly to the degree of contraction of the chromosomes used for the standard and, secondarily, to an intrinsic feature of bovine chromosomes, common to all the Bovidae, ie G-negative centromeres and telomeres. These problems have made it necessary to improve the Reading standard by using less contracted chromosomes and to adopt alternative banding techniques such as RBA and QFQ for sta