Separation of DNA Replication from the Assembly of Break-Competent Meiotic Chromosomes 英文参考文献.docVIP

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Separation of DNA Replication from the Assembly of Break-Competent Meiotic Chromosomes 英文参考文献.doc

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Separation of DNA Replication from the Assembly of Break-Competent Meiotic Chromosomes 英文参考文献

SeparationofDNAReplicationfromtheAssemblyof Break-CompetentMeioticChromosomes HannahG.Blitzblau1,2,ClaraS.Chan1,AndreasHochwagen2,3,StephenP.Bell1* 1DepartmentofBiology,HowardHughesMedicalInstitute,MassachusettsInstituteofTechnology,Cambridge,Massachusetts,UnitedStatesofAmerica,2Whitehead InstituteforBiomedicalResearch,NineCambridgeCenter,Cambridge,Massachusetts,UnitedStatesofAmerica,3DepartmentofBiology,NewYorkUniversity,NewYork, NewYork,UnitedStatesofAmerica Abstract Themeioticcelldivisionreducesthechromosomenumberfromdiploidtohaploidtoformgametesforsexualreproduction. Although much progress has been made in understanding meiotic recombination and the two meiotic divisions, the processesleadinguptorecombination,includingtheprolongedpre-meioticSphase(meiS)andtheassemblyofmeiotic chromosomeaxes,remainpoorlydefined.Wehaveusedgenome-wideapproachesinSaccharomycescerevisiaetomeasure the kinetics of pre-meiotic DNA replication and to investigate the interdependencies between replication and axis formation.WefoundthatreplicationinitiationwasdelayedforalargenumberoforiginsinmeiScomparedtomitosisand thatmeioticcellswerefarmoresensitivetoreplicationinhibition,mostlikelyduetothestarvationconditionsrequiredfor meiotic induction. Moreover, replication initiation was delayed even in the absence of chromosome axes, indicating replication timing is independent of the process of axis assembly. Finally, we found that cells were able to install axis components and initiate recombination on unreplicated DNA. Thus, although pre-meiotic DNA replication and meiotic chromosomeaxisformationoccurconcurrently,theyarenotstrictlycoupled.Thefunctionalseparationoftheseprocesses revealsamodularmethodofbuildingmeioticchromosomesandpredictsthatanycrosstalkbetweenthesemodulesmust occurthroughsuperimposedregulatorymechanisms. Citation:BlitzblauHG,ChanCS,HochwagenA,BellSP(2012)SeparationofDNAReplicationfromtheAssemblyofBreak-CompetentMeioticChromosomes.PLoS Genet8(5):e1002643.doi:10.1371/journal.pge