Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola 英文参考文献.docVIP

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Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola 英文参考文献.doc

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Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola 英文参考文献

Molecules 2012, 17, 2773-2783; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola Jan T?íska 1,*, Nadě?da Vrchotová 1, Julie Olejní?ková 2, Rudolf Jílek 1 and Radek Sotolá? 3 1 Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Center, Academy of Sciences of the Czech Republic, Brani?ovská 31, ?eské Budějovice 370 05, Czech Republic; E-Mails: vrchotova.n@czechglobe.cz (N.V.); jilek.r@czechglobe.cz (R.J.) 2 Laboratory of Plant Ecological Physiology, Global Change Research Center, Academy of Sciences of the Czech Republic, Zámek 136, Nové Hrady 373 33, Czech Republic; E-Mail: olejnickova.j@czechglobe.cz 3 Department of Viticulture, Faculty of Horticulture, Mendel University in Brno, Valtická 337, Lednice 691 44, Czech Republic; E-Mail: radek.sotolar@mendelu.cz * Author to whom correspondence should be addressed; E-Mail: triska.j@czechglobe.cz; Tel.: +420-387-775-619; Fax: +420-385-310-347. Received: 24 December 2011; in revised form: 27 February 2012 / Accepted: 28 February 2012 / Published: 6 March 2012 Abstract: A method for identification of highly fluorescent compounds in vine leaves infected by Plasmopara viticola was developed using reversed phase liquid chromatography with simultaneous diode array and fluorometric detection. Fluorescent compounds were extracted from leaves with a methanol-water mixture (70:30). Separation by HPLC was performed using a C18 column and gradient elution with water-acetonitrile mixtures (20–80% of acetonitrile). The main unknown fluorescent compound was identified by line spectral comparison with a standard obtained by UV photoisomerization of trans-resveratrol glucoside, and its structure was confirmed by liquid chromatography-mass spectrometry. Identification an