Sensitivity to electrical stimulation of human immunodeficiency virus type 1 and MAGIC-5 cells 英文参考文献.docVIP

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Sensitivity to electrical stimulation of human immunodeficiency virus type 1 and MAGIC-5 cells 英文参考文献.doc

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Sensitivity to electrical stimulation of human immunodeficiency virus type 1 and MAGIC-5 cells 英文参考文献

Kumagaietal.AMBExpress2011,1:23 /content/1/1/23 ORIGINAL OpenAccess Sensitivitytoelectricalstimulationofhuman immunodeficiencyvirustype1andMAGIC-5cells EtsukoKumagai1*,MasatoTominaga2andShinjiHarada3 Abstract Todeterminethesensitivitiestolowelectricalpotentialofhumanimmunodeficiencyvirustype1(HIV-1)andits targetcells,HIV-1andMAGIC-5cellsweredirectlystimulatedwithaconstantdirectcurrentpotentialof1.0V(vs. Ag/AgCl).HIV-1wasincubatedfor3hat37°Conapoly-L-lysine-coatedindium-tinoxideelectrode,andthen stimulatedbyanelectricalpotential.MAGIC-5cellswereseededontotheelectricallystimulatedHIV-1andcultured for3daysat37°C.HIV-1-infectedcellsweremeasuredbymultinuclearactivationviaagalactosidaseindicatorassay. MAGIC-5cellswerealsostimulatedbyanelectricalpotentialof1.0V;celldamage,proliferationandapoptosiswere evaluatedbytrypanbluestaining,cellcountingandinsituapoptosisdetection,respectively.HIV-1wasfoundtobe damagedtoagreaterextentbyelectricalstimulationthanthecells.Inparticular,afterapplicationofa1.0-V potentialfor3min,HIV-1LAI andHIV-1KMT infectionwereinhibitedbyabout90%,butchangesincelldamage, proliferationandapoptosiswerevirtuallyundetectable.TheseresultssuggestedthatHIV-1issignificantlymore susceptibletolowelectricalpotentialthancells.Thisfindingcouldformthebasisofanoveltherapeuticstrategy againstHIV-1infection. Keywords:HIV-1infectivity,electricalstimulation,indium-tinoxide,poly-L-lysine Introduction 90% of circulating HIV-1 isolates, were identified (Wu Infection with human immunodeficiency virus type 1 et al. 2010,; Zhou et al. 2010). The therapeutic use of (HIV-1), the causative agent of acquired immunodefi- multiplebroadlyneutralizinghumanmonoclonalantibo- ciency syndrome (AIDS), leads to depressed cellular dies to HIV-1 would therefore be expected to block immunity andcan result in co-infection with opportu- HIV-1 infection. However, effective vaccines based on nisticpathogensandseveredisease(Gottlieetal.1981,; thisstrategy areyettobedeveloped andmuchintere