Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR) 英文参考文献.docVIP
- 1、本文档共9页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR) 英文参考文献
Sensors 2010, 10, 2045-2053; doi:10.3390/s100302045
OPEN ACCESS
sensors
ISSN 1424-8220
/journal/sensors
Article
Signal Amplification by Enzymatic Reaction in an
Immunosensor Based on Localized Surface Plasmon
Resonance (LSPR)
Tae-Han Lee 1,2, Seung-Woo Lee 1, Ji-Ae Jung 1,2, Junhyoung Ahn 1,2, Min-Gon Kim 1,2
and
Yong-Beom Shin 1,2,
*
1
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea;
E-Mail: swlee71@kribb.re.kr (S.-W.L)
2
University of Science and Technology (UST), 305-333 Daejeon, Korea;
E-Mails: t2taehan@ (T.-H.L.); jiae86@kribb.re.kr (J.-A.J.); ajh@kribb.re.kr (J.A);
mgkim@kribb.re.kr (M.-G.K.)
* Author to whom correspondence should be addressed; E-Mail: ybshin@kribb.re.kr;
Tel.: +82-42-860-4449, Fax: +82-42-879-8594.
Received: 20 January 2010; in revised form: 15 February 2010 / Accepted: 4 March 2010 /
Published: 12 March 2010
Abstract: An enzymatic reaction was employed as a means to enhance the sensitivity of an
immunosensor based on localized surface plasmon resonance (LSPR). The reaction occurs
after intermolecular binding between an antigen and an antibody on gold nano-island (NI)
surfaces. For LSPR sensing, the gold NI surface was fabricated on glass substrates using
vacuum evaporation and heat treatment. The interferon-? (IFN-?) capture antibody was
immobilized on the gold NIs, followed by binding of IFN-? to the antibody. Subsequently, a
biotinylated antibody and a horseradish peroxidase (HRP) conjugated with avidin were
simultaneously introduced. A solution of 4-chloro-1-naphthol (4-CN) was then used for
precipitation; precipitation was the result of the enzymatic reaction catalyzed the HRP on
gold NIs. The LSPR spectra were obtained after each binding process. Using this method,
the enzyme-catalyzed precipitation reaction on the gold NI surface was found to effectively
amplify the change in the signal of the LSPR immunosensor after intermolecula
您可能关注的文档
- Shrink-Induced Superhydrophobic and Antibacterial Surfaces in Consumer Plastics 英文参考文献.doc
- Should Younger Siblings of Peanut-Allergic Children Be Assessed by an Allergist before Being Fed Peanut 英文参考文献.doc
- Shrinking the Metabolic Solution Space Using Experimental Datasets 英文参考文献.doc
- Shunting Inhibition Controls the Gain Modulation Mediated by Asynchronous Neurotransmitter Release in Early Development 英文参考文献.doc
- Shut Down, Don't Stress Out 英文参考文献.doc
- Shuffling of cis-regulatory elements is a pervasive feature of the vertebrate lineage 英文参考文献.doc
- Sib-recruitment for studying migration and its impact on obesity and diabetes 英文参考文献.doc
- Sialoglycosylation of RBC in Visceral Leishmaniasis Leads to Enhanced Oxidative Stress, Calpain-Induced Fragmentation of Spectrin and Hemolysis 英文参考文献.doc
- Sialoadhesin Expressed on IFN-Induced Monocytes Binds HIV-1 and Enhances Infectivity 英文参考文献.doc
- Sialome of a Generalist Lepidopteran Herbivore Identification of Transcripts and Proteins from Helicoverpa armigera Labial Salivary Glands 英文参考文献.doc
- 2025福建漳州新园轻工实业招聘2人备考试题及答案解析.docx
- 纺丝工岗位责任制.doc
- 2025江西南昌联帆环境工程有限公司派遣招聘备考试题及答案解析.docx
- 纺丝工(纺丝凝固浴液配制工)上岗培训教案.doc
- 2025安徽池州东至县洋湖镇人民政府招聘编外消防人员2人备考试题及答案解析.docx
- 2025福建漳州云霄立人学校诚聘中学教师3人备考试题及答案解析.docx
- 飞行教学讲评课件.ppt
- 2025黑龙江齐齐哈尔克东县公益性岗位人员招聘56人备考试题及答案解析.docx
- 2025贵州黔西南州贞丰县总工会招聘社会工作者5人备考试题及答案解析.docx
- 2025广东湛江雷州市卫生健康局下属事业单位招聘卫生事业人员65人备考试题及答案解析.docx
最近下载
- 腹腔引流管脱管应急预案.pptx VIP
- 呼吸衰竭最新治疗指南解读PPT课件.pptx VIP
- 呼吸衰竭最新治疗指南解读PPT课件.pptx VIP
- 辟谷养身:12.空腹力革命.pdf VIP
- 施工组织设计主要经济指标.pptx VIP
- 2023年ISO15189 医学实验室管理体系全套表格.docx VIP
- DLT5210-2021版第一部分土建工程(热力系统土建工程质量验收)可编辑表格.docx VIP
- 10000字在学校挨机器人板子的作文.docx VIP
- 《A水利枢纽的拱坝设计中拱坝应力分析计算案例》3000字.docx VIP
- 2025年河北承德市中小学教师招聘考试试卷带答案.docx VIP
文档评论(0)