Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR) 英文参考文献.docVIP

Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR) 英文参考文献.doc

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Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR) 英文参考文献

Sensors 2010, 10, 2045-2053; doi:10.3390/s100302045 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR) Tae-Han Lee 1,2, Seung-Woo Lee 1, Ji-Ae Jung 1,2, Junhyoung Ahn 1,2, Min-Gon Kim 1,2 and Yong-Beom Shin 1,2, * 1 Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea; E-Mail: swlee71@kribb.re.kr (S.-W.L) 2 University of Science and Technology (UST), 305-333 Daejeon, Korea; E-Mails: t2taehan@ (T.-H.L.); jiae86@kribb.re.kr (J.-A.J.); ajh@kribb.re.kr (J.A); mgkim@kribb.re.kr (M.-G.K.) * Author to whom correspondence should be addressed; E-Mail: ybshin@kribb.re.kr; Tel.: +82-42-860-4449, Fax: +82-42-879-8594. Received: 20 January 2010; in revised form: 15 February 2010 / Accepted: 4 March 2010 / Published: 12 March 2010 Abstract: An enzymatic reaction was employed as a means to enhance the sensitivity of an immunosensor based on localized surface plasmon resonance (LSPR). The reaction occurs after intermolecular binding between an antigen and an antibody on gold nano-island (NI) surfaces. For LSPR sensing, the gold NI surface was fabricated on glass substrates using vacuum evaporation and heat treatment. The interferon-? (IFN-?) capture antibody was immobilized on the gold NIs, followed by binding of IFN-? to the antibody. Subsequently, a biotinylated antibody and a horseradish peroxidase (HRP) conjugated with avidin were simultaneously introduced. A solution of 4-chloro-1-naphthol (4-CN) was then used for precipitation; precipitation was the result of the enzymatic reaction catalyzed the HRP on gold NIs. The LSPR spectra were obtained after each binding process. Using this method, the enzyme-catalyzed precipitation reaction on the gold NI surface was found to effectively amplify the change in the signal of the LSPR immunosensor after intermolecula

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