Specific eradication of HIV-1 from infected cultured cells 英文参考文献.docVIP

Levinetal.AIDSResearchandTherapy2010,7:31 /content/7/1/31 SHORT REPORT OpenAccess SpecificeradicationofHIV-1frominfected culturedcells AviadLevin1,ZviHayouka2,AssafFriedler2,AbrahamLoyter1* Abstract AcorrelationbetweenincreaseintheintegrationofHumanImmunodeficiencyvirus-1(HIV-1)cDNAandcelldeath waspreviouslyestablished.Hereweshowthatcombinationofpeptidesthatstimulateintegrationtogetherwith theproteaseinhibitorRo31-8959causedapoptoticcelldeathofHIVinfectedcellswithtotalexterminationofthe virus.Thiscombinationdidnothaveanyeffectonnon-infectedcells.Thusitappearsthatcelldeathispromoted onlyintheinfectedcells.Itisourviewthattheresultsdescribedinthisworksuggestanovelapproachtospecifi- callypromotedeathofHIV-1infectedcellsandthusmayeventuallybedevelopedintoanewandgeneral anti-viraltherapy. Findings numerous copies of unintegrated viral cDNA [22,24]. No cure or vaccine are currently available for the Recently we have shown that this restriction is due to humanimmunodeficiencyvirustype1(HIV-1)infection inhibitionoftheviralINactivityaswellasofitsnuclear aswellasfortheresultingAcquiredImmunodeficiency importbyanearlyexpressedviralRevproteinfollowing syndrome(AIDS)[1].However,anhighlyactiveantire- Rev-IN interaction [25-30]. Disruption of the Rev-IN troviraltherapy(HAART),whichblockstheactivitiesof complexbyIN-derivedcellpermeablepeptides,suchas theviralreversetranscriptase andproteaseandinhibits theINS[31] andINrs[28], results inmulti-integration the virus-host fusion process, is presently used [2,3]. of the viral cDNA [26,28,31]. Previous findings have The HAART transforms the infection process into a shown that multi-integration of viral DNA in AIDS chronicdisease[4-6].Furthermore,theriskofinfection patients may lead to host genome instability [32]. cansignificantlybereducediftheHAARTtreatmentis Indeed, acorrelation betweenpromotionofmulti-inte- givenrightafterexposuretothevirus[7]. gration and increase in cell death was demonstrate