Stallion spermatozoa selected by single layer centrifugation are capable of fertilization after storage for up to 96？h at 6°C prior to artificial insemination 英文参考文献.docVIP

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Stallion spermatozoa selected by single layer centrifugation are capable of fertilization after storage for up to 96？h at 6°C prior to artificial insemination 英文参考文献.doc

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Stallion spermatozoa selected by single layer centrifugation are capable of fertilization after storage for up to 96？h at 6°C prior to artificial insemination 英文参考文献

Lindahletal.ActaVeterinariaScandinavica2012,54:40 /content/54/1/40 RESEARCH OpenAccess Stallionspermatozoaselectedbysinglelayer centrifugationarecapableoffertilizationafter storageforupto96hat6°Cpriortoartificial insemination JohannaLindahl1,Anne-MarieDalin1,GesaStuhtmann2andJaneMMorrell1* Abstract Background:Oneofthechallengesfacedbyequinebreedersisensuringdeliveryofgoodqualitysemendosesfor artificialinseminationwhenthemareisduetoovulate.SingleLayerCentrifugation(SLC)hasbeenshowntoselect morphologicallynormalspermatozoawithintactchromatinandgoodprogressivemotilityfromtherestofthe ejaculate,andtoprolongthelifeoftheseselectedspermatozoainvitro.Theobjectiveofthepresentstudywasa proofofconcept,todeterminewhetherfertilizingabilitywasretainedinSLC-selectedspermatozoaduring prolongedstorage. Findings:SixteenmareswereinseminatedwithSLC-selectedspermdosesthathadbeencooledandstoredat6°C for48h,72hor96h.Embryoswereidentifiedin11maresbyultrasoundexamination16–18daysafterpresumed ovulation. Conclusion:SLC-selectedstallionspermatozoastoredforupto96harecapableoffertilization. Keywords:Singlelayercentrifugation,Storedstallionspermatozoa,Fertilizingcapability,Artificialinsemination Background In Europe, the most usual method of equine artificial insemination (AI) is with cooled semen doses, with AI doses would be beneficial to the equine breeding industry. SingleLayerCentrifugation(SLC)isacolloidcentrifu- taking place within 24-36 h of semen collection. How- gation technique designed to select spermatozoa with ever, there can be problems with delivery of cooled good motility, membrane integrity, normal morphology semen on the day when it is required, particularly over and intact chromatin from the rest of the ejaculate [2]. the weekends and during public holidays. Although Progressive motility is prolonged in SLC-selected sperm some stallion ejaculates will retain satisfactory sperm samples, at least up to 72 h and often longer [3,4,5]. quality for 24-36 h [1], others do