Thermus thermophilus Strains Active in Purine Nucleoside Synthesis 英文参考文献.docVIP

Molecules 2009, 14, 1279-1287; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Thermus thermophilus Strains Active in Purine Nucleoside Synthesis Marcos Almendros 1,2, José-Vicente Sinisterra Gago 1,3,* and José Berenguer Carlos 2 1 Biotransformations Group. Servicio de Biotransformaciones Industriales. Parque Científico de Madrid. C/ Santiago Grisolia no 2. 28760 Tres Cantos. Madrid, Spain 2 Centro de Biología Molecular Severo Ochoa, UAM 28049 Cantoblanco. Madrid. Spain. 3 Department of Organic Pharmaceutical Chemistry. Faculty of Pharmacy. Universidad Complutense, 28040 Madrid, Spain * Author to whom correspondence should be addressed; E-mail: jose.sinisterra@fpcm.es; Tel.: 34-91- 8040581; Fax: 34-91-8037289. Received: 26 December 2008; in revised form: 25 February 2009 / Accepted: 11 March 2009 / Published: 24 March 2009 Abstract: Several strains of Thermus thermophilus were tested in order to detect purine nucleoside synthase activity using pyrimidine nucleosides as the sugar-donor and adenine or hypoxanthine as bases. High productivity values (t =1 hr) were obtained while completely avoiding adenosine-deaminase degradation of the products. N-2-deoxy- ribosyltransferase activity is described for the first time in hyperthermophilic bacteria. Keywords: Biocatalysis; Nucleoside synthesis; Thermus thermophilus. Introduction Thermophiles are a group of microorganisms that grow under extreme temperature conditions. These microorganisms offer useful enzymes to expand the range of reaction conditions suitable for biocatalysis. In this way some interesting enzymes have been described such as esterases, lipases, proteases, alcohol dehydrogenases, etc. [1,2]. Nucleoside analogues are labile and polyfunctional molecules which chemical synthesis requires several protection/deprotection steps [3]. These compounds have a wide