Throwing the dice for the diagnosis of vaginal complaints 英文参考文献.docVIP

Annals of Clinical Microbiology and Antimicrobials BioMedCentral Research Open Access Throwing the dice for the diagnosis of vaginal complaints? Andreas Schwiertz*1, David Taras2, Kerstin Rusch1 and Volker Rusch1 Address: 1Institute for microecology, Herborn, Germany and 2Institute of Animal Nutrition, Faculty of Veterinary Medicine, Free University Berlin, Germany Email: Andreas Schwiertz* - andreas.schwiertz@mikrooek.de; David Taras - dtaras@zedat.fu-berlin.de; Kerstin Rusch - kerstin.rusch@mikrooek.de; Volker Rusch - volker.rusch@mikrooek.de * Corresponding author Published: 17 February 2006 Received: 10 November 2005 Accepted: 17 February 2006 Annals of Clinical Microbiology and Antimicrobials 2006, 5:4 doi:10.1186/1476-0711-5-4 This article is available from: /content/5/1/4 ? 2006 Schwiertz et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Backround: Vaginitis is among the most common conditions women are seeking medical care for. Although these infections can easily be treated, the relapse rate is high. This may be due to inadequate use of the diagnostic potential. Methods: We evaluated the misjudgement rate of the aetiology of vaginal complaints. A total of 220 vaginal samples from women with a vaginal complaint were obtained and analysed for numbers of total lactobacilli, H2O2-producing lactobacilli, total aerobic cell counts and total anaerobic cell counts including bifidobacteria, Bacteroides spp., Prevotella spp. Additionally, the presence of Atopobium vaginae, Gardnerella vaginalis, Candida spp. and Trichomonas vaginalis was evaluated by DNA-hybridisation using the PCR and Affirm VPIII Microbial Identification Test, respectively. Results: The participating