Towards a Guided Regeneration of Renal Tubules at a Polyester Interstitium 英文参考文献.docVIP

Materials 2010, 3, 2369-2392; doi:10.3390/ma3042369 OPEN ACCESS materials ISSN 1996-1944 /journal/materials Review Towards a Guided Regeneration of Renal Tubules at a Polyester Interstitium Will W. Minuth *, Lucia Denk and Anne Glashauser Department of Molecular and Cellular Anatomy, University of Regensburg, University Street 31, 93053 Regensburg, Germany; E-Mails: lucia.denk@vkl.uni-regensburg.de (L.D.); anne.glashauser@vkl.uni-regensburg.de (A.G.) * Author to whom correspondence should be addressed; E-Mail: will.minuth@vkl.uni-regensburg.de; Tel.: +49-941-943-2876, Fax: +49-941-943-2868. Received: 16 January 2010; in revised form: 15 March 2010 / Accepted: 26 March 2010 / Published: 26 March 2010 Abstract: Stem/progenitor cells are promising candidates for a therapy of renal failure. However, sound knowledge about implantation and regeneration is lacking. Therefore, mechanisms leading from stem/progenitor cells into tubules are under research. Renal stem/progenitor cells were isolated from neonatal rabbit kidney and mounted between layers of polyester fleece. It creates an artificial interstitium and replaces coating by extracellular matrix proteins. Tubulogenic development is induced by aldosterone. Electron microscopy illuminates growth of tubules in close vicinity to polyester fibers. Tubules contain a differentiated epithelium. The spatial extension of tubules opens a new strategy for testing morphogenic drugs and biocompatible fleece materials. Keywords: polyester fleece; artificial interstitium; kidney; stem/progenitor cells; regeneration; aldosterone 1. Introduction 1.1. Limited regeneration of renal parenchyma The capability of parenchyma regeneration is limited in patients with chronic or acute renal failure [1–3]. In view of this clinical background, the question arose as to which molecular processes hamper a diseased kidney to form new parenchyma [4–6]. Du