Transcript profiling reveals complex auxin signalling pathway and transcription regulation involved in dedifferentiation and redifferentiation during somatic embryogenesis in cotton 英文参考文献.docVIP

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Transcript profiling reveals complex auxin signalling pathway and transcription regulation involved in dedifferentiation and redifferentiation during somatic embryogenesis in cotton 英文参考文献.doc

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Transcript profiling reveals complex auxin signalling pathway and transcription regulation involved in dedifferentiation and redifferentiation during somatic embryogenesis in cotton 英文参考文献

Yangetal.BMCPlantBiology2012,12:110 /1471-2229/12/110 RESEARCH ARTICLE OpenAccess Transcriptprofilingrevealscomplexauxin signallingpathwayandtranscriptionregulation involvedindedifferentiationandredifferentiation duringsomaticembryogenesisincotton XiyanYang,XianlongZhang*,DaojunYuan,FangyanJin,YunchaoZhangandJiaoXu Abstract Background:Somaticembryogenesis(SE),bywhichsomaticcellsofhigherplantscandedifferentiateand reorganizeintonewplants,isanotableillustrationofcelltotipotency.However,theprecisemolecularmechanisms regulatingSEremainunclear.Tocharacterizethemoleculareventsofthisuniqueprocess,transcriptomeanalysis,in combinationwithbiochemicalandhistologicalapproaches,wereconductedincotton,atypicalplantspeciesinSE. Genome-wideprofilingofgeneexpressionallowedtheidentificationofnovelmolecularmarkerscharacteristicof thisdevelopmentalprocess. Results:RNA-Seqwasusedtoidentify5,076differentiallyexpressedgenesduringcottonSE.Expressionprofileand functionalassignmentsofthesegenesindicatedsignificanttranscriptionalcomplexityduringthisprocess, associatedwithmorphological,histologicalchangesandendogenousindole-3-aceticacid(IAA)alteration. Bioinformaticsanalysisshowedthatthegeneswereenrichedforbasicprocessessuchasmetabolicpathwaysand biosynthesisofsecondarymetabolites.Unigeneswereabundantforthefunctionsofproteinbindingandhydrolase activity.Transcriptionfactor–encodinggeneswerefoundtobedifferentiallyregulatedduringSE.Thecomplex pathwaysofauxinabundance,transportandresponsewithdifferentiallyregulatedgenesrevealedthatthe auxin-relatedtranscriptsbelongedtoIAAbiosynthesis,indole-3-butyricacid(IBA)metabolism,IAAconjugate metabolism,auxintransport,auxin-responsiveprotein/indoleaceticacid-inducedprotein(Aux/IAA),auxinresponse factor(ARF),smallauxin-upRNA(SAUR),Aux/IAAdegradation,andotherauxin-relatedproteins,whichallowan intricatesystemofauxinutilizationtoachievemultiplepurposesinSE.Quantitativereal-timePCR(qRT-PCR)was performedonselectedgeneswithdifferentexpressionpatternsandfunctional