Transcription regulation no holy grail, but many treasures 英文参考文献.docVIP

Meeting report Transcription regulation: no holy grail, but many treasures Nicola A Hawkes and Patrick Varga-Weisz Address: Babraham Institute, Babraham, Cambridge CB3 4AT, UK. Correspondence: Patrick Varga-Weisz. E-mail: patrick.varga-weisz@bbsrc.ac.uk Published: 29 June 2005 Genome Biology 2005, 6:334 (doi:10.1186/gb-2005-6-7-334) The electronic version of this article is the complete one and can be found online at /2005/6/7/334 ? 2005 BioMed Central Ltd the Rad26-Def1 complex as a mechanism for dealing with A report of the Biochemical Society Annual Symposium ‘Transcription UK’, London, UK, 13-15 April 2005. this problem and has reconstituted the reaction in vitro. After RNA polymerase has successfully transcribed through a gene, termination of transcription becomes an important regulatory step that may, in turn, impact on subsequent tran- scriptional reinitiation. Analysis of transcriptional termination in the humanβ-globin gene has revealed the phenomenon of co-transcriptional cleavage (CoTC). Cleavage within the pre- messenger RNA, downstream of the poly(A) addition site, is critical for efficient termination and involves an RNA self- cleaving activity. An important question is whether this is a general phenomenon. Alexandre Akoulitchev (University of Oxford) described his group’s use of bioinformatics to iden- tify a novel regulatory element, found both 5? and 3? of many genes, which they have termed ‘Checkpoint Charlie’, and which is believed to play a part in the regulation of CoTC and the definition of transcription borders. Nick Proudfoot (Uni- versity of Oxford) described his lab’s work, which has revealed another CoTC event in the mouse albumin termina- tor sequence. Given that Proudfoot’s work implicated ATP- dependent nucleosome-remodeling factors in termination, it will be interesting to see whether they regulate termination via Checkpoint Charlie, maybe by mediati