Transcriptome analysis of haploid male gametophyte development in Arabidopsis 英文参考文献.docVIP

Open Access Research 2004HonysVolumeand5, ITwellssue 11, Article R85 Transcriptome analysis of haploid male gametophyte development in Arabidopsis David Honys*? and David Twell? Addresses: *Institute of Experimental Botany AS CR, Rozvojová 135, CZ-165 02, Praha 6, Czech Republic. ?Department of Plant Physiology, Faculty of Sciences, Charles University, Vini?ná 5, CZ-128 44, Praha 2, Czech Republic. ?Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, UK. Correspondence: David Honys. E-mail: honys@ueb.cas.cz. David Twell. E-mail: twe@leicester.ac.uk Published: 27 October 2004 Genome Biology 2004, 5:R85 Received: 10 August 2004 Revised: 17 September 2004 Accepted: 28 September 2004 The electronic version of this article is the complete one and can be found online at /2004/5/11/R85 ? 2004 Honys and Twell; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. TranscriptomeoppenAs ttrhaendscoroirptanalysisomdetaanilaeofldyshaploidsitsuodfiems aomalelfetghaemgametophytereegtouplahtyotreydpeavdevelopmenttehlwopamyseinntvionlvAinerdaArabidopsis.bidopsis/p uncovers distinct temporal classes of gene expression and Abstract Background: The haploid male gametophyte generation of flowering plants consists of two- or three-celled pollen grains. This functional specialization is thought to be a key factor in the evolutionary success of flowering plants. Moreover, pollen ontogeny is also an attractive model in which to dissect cellular networks that control cell growth, asymmetric cell division and cellular differentiation. Our objective, and an essential step towards the detailed understanding of these processes, was to comprehensively define the male haploid