Transcriptome analysis of germinating maize kernels exposed to smoke-water and the active compound KAR1 英文参考文献.docVIP
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Transcriptome analysis of germinating maize kernels exposed to smoke-water and the active compound KAR1 英文参考文献
Soósetal.BMCPlantBiology2010,10:236
/1471-2229/10/236
RESEARCH ARTICLE
OpenAccess
Transcriptomeanalysisofgerminatingmaize
kernelsexposedtosmoke-waterandtheactive
compoundKAR1
VilmosSoós1?,EndreSebestyén1?,AngélaJuhász1,MarnieELight2,LadislavKohout3,GabriellaSzalai4,
JúliaTandori4,JohannesVanStaden2,ErvinBalázs1*?
Abstract
Background:Smokereleasedfromburningvegetationfunctionsasanimportantenvironmentalsignalpromoting
thegerminationofmanyplantspeciesfollowingafire.Itnotonlypromotesthegerminationofspeciesfromfire-
pronehabitats,butseveralspeciesfromnon-fire-proneareasalsorespond,includingsomecrops.Thegermination
stimulatoryactivitycanlargelybeattributedtothepresenceofahighlyactivebutenolidecompound,3-methyl-2H-
furo[2,3-c]pyran-2-one(referredtoaskarrikin1orKAR1),thathaspreviouslybeenisolatedfromplant-derived
smoke.SeveralhypotheseshavearisenregardingthemolecularbackgroundofsmokeandKAR1action.
Results:Inthispaperwedemonstratethatalthoughsmoke-waterandKAR1treatmentofmaizekernelsresultina
similarphysiologicalresponse,thegeneexpressionandtheproteinubiquitinationpatternsarequitedifferent.
Treatmentwithsmoke-waterenhancedtheubiquitinationofproteinsandactivatedprotein-degradation-related
genes.ThiseffectwascompletelyabsentfromKAR1-treatedkernels,inwhichaspecificaquaporingenewas
distinctlyupregulated.
Conclusions:Ourfindingsindicatethatthearrayofbioactivecompoundspresentinsmoke-waterforman
environmentalsignalthatmayacttogetheringerminationstimulation.Itishighlypossiblethatthesmoke/KAR1
’signal’isperceivedbyareceptorthatissharedwiththesignaltransductionsystemimpliedinperceiving
environmentalcues(especiallystressesandlight),orsomekindofspecializedreceptorexistsinfire-proneplant
specieswhichdivergedfromamoregeneralonepresentinacommonancestor,andalsofoundinnonfire-prone
plantsallowingforasomewhatweakerbutstillsignificantresponse.Besidestheirobvioususeinagricultural
practices,smokeandKAR1canbeusedinstudiestogainfurtherinsightintothetranscriptionalchangesduring
germination.
Backg
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