Translational regulation of gene expression 英文参考文献.docVIP

Meeting report Translational regulation of gene expression Stephanie Kervestin and Nadia Amrani Address: Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655-0122, USA. Correspondence: Nadia Amrani. E-mail: nadia.amrani@ Published: 25 November 2004 Genome Biology 2004, 5:359 The electronic version of this article is the complete one and can be found online at /2004/5/12/359 ? 2004 BioMed Central Ltd translocation and, together, these structural data highlighted A report on the Cold Spring Harbor Laboratory meeting the dynamic nature of ribosome structure during the differ- ‘Translational Control’, Cold Spring Harbor, USA, 7-12 ent steps of translation and prompted the audience to September 2004. ponder which conformational changes are rate-limiting during translation. There have been major breakthroughs in recent years in understanding both the mechanism of mRNA translation and its control. High-resolution structures have revealed the ribosome’s role in the decoding process and the ribozyme activity of its peptidyl transferase center. The importance of post-transcriptional mechanisms in the regulation of gene expression is also much better appreciated today. The 2004 Structural analysis of the eukaryotic ribosome when associ- ated with translation factors has also brought new insights. In eukaryotes, initiation of translation is generally depen- dent on the presence of a 5? cap structure on the messenger RNA. Cap-dependent translation initiation is a complex process, facilitated by a large number of initiation factors (eIFs) that form a complicated network of cooperative inter- actions with the 40S ribosomal subunit. John McCarthy (Manchester Interdisciplinary Biocentre, UK) reported cryo- electron microscopy (cryo-EM) reconstructions, which indi- cate that binding of eIF1A to the 40