Transcriptome analysis of Sinorhizobium meliloti during symbiosis 英文参考文献.docVIP

Open Access Research Transcriptome analysis of Sinorhizobium meliloti during symbiosis Frédéric Ampe, Ern? Kiss, Frédérique Sabourdy and Jacques Batut Address: Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, UMR 215 Centre National de la Recherche Scientifique - Institut National de la Recherche Agronomique, BP27-31326 Castanet-Tolosan cedex, France. Correspondence: Jacques Batut. E-mail: jbatut@toulouse.inra.fr Published: 31 January 2003 Received: 19 September 2002 Revised: 14 November 2002 Accepted: 18 December 2002 Genome Biology 2003, 4:R15 The electronic version of this article is the complete one and can be found online at /2003/4/2/R15 ? 2003 Ampe et al.; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the articles original URL. Abstract Background: Rhizobia induce the formation on specific legumes of new organs, the root nodules, as a result of an elaborated developmental program involving the two partners. In order to contribute to a more global view of the genetics underlying this plant-microbe symbiosis, we have mined the recently determined Sinorhizobium meliloti genome sequence for genes potentially relevant to symbiosis. We describe here the construction and use of dedicated nylon macroarrays to study simultaneously the expression of 200 of these genes in a variety of environmental conditions, pertinent to symbiosis. Results: The expression of 214 S. meliloti genes was monitored under ten environmental conditions, including free-living aerobic and microaerobic conditions, addition of the plant symbiotic elicitor luteolin, and a variety of symbiotic conditions. Five new genes induced by luteolin have been identified as well as nine new genes induced in