Transcriptomic analysis of pluripotent stem cells insights into health and disease 英文参考文献.docVIP

Yeo and Ng Genome Medicine 2011,3:68 /content/3/10/68 RE VIE W Transcriptomic analysis of pluripotent stem cells: insights into health and disease Jia-Chi Yeo and Huck-Hui Ng * 1,2 1,2,3,4,5, types, termed ‘pluripotency’, allows researchers to study early mammalian development in an arti?cial setting and o?ers opportunities for regenerative medicine, whereby ESCs could generate clinically relevant cell types for tissue repair. However, this same malleability of ESCs also renders it a challenge to obtain in vitro di?erentiation of ESCs to speci?c cell types at high e?cacy. erefore, harnessing the full potential of ESCs requires an in-depth understanding of the factors and mechanisms regulating ESC pluripotency and cell lineage decisions. Abstract Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) hold tremendous clinical potential because of their ability to self-renew, and to dierentiate into all cell types of the body. This unique capacity of ESCs and iPSCs to form all cell lineages is termed pluripotency. While ESCs and iPSCs are pluripotent and remarkably similar in appearance, whether iPSCs truly resemble ESCs at the molecular level is still being debated. Further research is therefore needed to resolve this issue before iPSCs may be safely applied in humans for cell therapy or regenerative medicine. Nevertheless, the use of iPSCs as an in vitro human genetic disease model has been useful in studying the molecular pathology of complex genetic diseases, as well as facilitating genetic or drug screens. Here, we review recent progress in transcriptomic approaches in the study of ESCs and iPSCs, and discuss how deregulation of these pathways may be involved in the development of disease. Finally, we address the importance of these advances for developing new therapeutics, and the future challenges facing the clinical application of ESCs and iPSCs. Early