Transcriptomic analysis of tomato carpel development reveals alterations in ethylene and gibberellin synthesis during pat3pat4 parthenocarpic fruit set 英文参考文献.docVIP

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Transcriptomic analysis of tomato carpel development reveals alterations in ethylene and gibberellin synthesis during pat3pat4 parthenocarpic fruit set 英文参考文献.doc

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Transcriptomic analysis of tomato carpel development reveals alterations in ethylene and gibberellin synthesis during pat3pat4 parthenocarpic fruit set 英文参考文献

BMC Plant Biology BioMedCentral Research article Open Access Transcriptomic analysis of tomato carpel development reveals alterations in ethylene and gibberellin synthesis during pat3/pat4 parthenocarpic fruit set Laura Pascual, Jose M Blanca, Joaquin Ca?izares*? and Fernado Nuez? Address: Instituto de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Universidad Politécnica de Valencia, Camino de Vera s/ n, 46022 Valencia, Spain Email: Laura Pascual - laupasba@upvnet.upv.es; Jose M Blanca - jblanca@btc.upv.es; Joaquin Ca?izares* - jcanizares@upvnet.upv.es; Fernado Nuez - fnuez@btc.upv.es * Corresponding author ?Equal contributors Published: 29 May 2009 Received: 28 July 2008 Accepted: 29 May 2009 BMC Plant Biology 2009, 9:67 doi:10.1186/1471-2229-9-67 This article is available from: /1471-2229/9/67 ? 2009 Pascual et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Tomato fruit set is a key process that has a great economic impact on crop production. We employed the Affymetrix GeneChip Tomato Genome Array to compare the transcriptome of a non-parthenocarpic line, UC82, with that of the parthenocarpic line RP75/59 (pat3/pat4 mutant). We analyzed the transcriptome under normal conditions as well as with forced parthenocarpic development in RP75/59, emasculating the flowers 2 days before anthesis. This analysis helps to understand the fruit set in tomato. Results: Differentially expressed genes were extracted with maSigPro, which is designed for the analysis of single and multiseries time course microarray experiments. 2842 genes showed changes throughout normal carpel development and fruit set. Most of them showed a change of expression at or after an