Triterpenes from Stachyurus himalaicus var. himalaicus Hook. f. et Thoms. ex Benth 英文参考文献.docVIP

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Triterpenes from Stachyurus himalaicus var. himalaicus Hook. f. et Thoms. ex Benth 英文参考文献.doc

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Triterpenes from Stachyurus himalaicus var. himalaicus Hook. f. et Thoms. ex Benth 英文参考文献

Molecules 2010, 15, 2096-2102; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Triterpenes from Stachyurus himalaicus var. himalaicus Hook. f. et Thoms. ex Benth Yun-Song Wang 1, Rong Huang 1, Ning-Zhong Li 2, Hong-Yi Xu 3 and Jing-Hua Yang 1,* 1 Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming 650091, China; E-Mail: wangys@ (Y.-S.W.) 2 Xin Feng Hospital of Xi-Shang District, Kunming 650100, China; E-Mail: liningzhong@ (N.Z.L.) 3 Department of Public Security of Yunnan Province, Kunming 650031, China; E-Mail: hongyix@ (H-Y.X.) ? Author to whom correspondence may be addressed; E-Mail: yangjh@; Tel: +86-871-6598387; Fax: +86-871-5035538. Received: 14 January 2010; in revised form: 24 February 2010 / Accepted: 22 March 2010 / Published: 24 March 2010 Abstract: Four triterpenes 1–4, including a new naturally occurring oleanane-type triterpene 1, were isolated by a multi-step chromatography procedure from the leaves and twigs of Stachyurus himalaicus var. himalaicus Hook. f. et Thoms.ex Benth. The structures of the compounds were elucidated by spectroscopic methods, including HRESIMS, 1H- NMR, 13C-NMR, DEPT, HMQC, HMBC and NOESY spectra. All the isolated compounds were evaluated for their in vitro cytotoxic activities against human Hela cell line. Keywords: stachyuraceae; Stachyurus himalaicus var. himalaicus Hook. f. et Thoms. Ex Benth; triterpenes; stachlic acid C 1. Introduction Stachyurus, the only genus in the Stachyuraceae family, consists of 10 species distributed in Eastern Asia [1]. Early studies regarding the chemical constituents of Stachyurus revealed the presence of ellagitannins, hydrolysable tannins and complex tannins [2–4]. Stachyurus himalaicus var. himalaicus Molecules 2010, 15 2097 Hook. f. et Thoms. ex Benth is known as “Tong-Cao” in Chinese folk