Uncovering the Arabidopsis thaliana nectary transcriptome investigation of differential gene expression in floral nectariferous tissues 英文参考文献.docVIP

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Uncovering the Arabidopsis thaliana nectary transcriptome investigation of differential gene expression in floral nectariferous tissues 英文参考文献.doc

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Uncovering the Arabidopsis thaliana nectary transcriptome investigation of differential gene expression in floral nectariferous tissues 英文参考文献

BMC Plant Biology BioMedCentral Research article Open Access Uncovering the Arabidopsis thaliana nectary transcriptome: investigation of differential gene expression in floral nectariferous tissues Brian W Kram?1, Wayne W Xu?2 and Clay J Carter*1 Address: 1Department of Biology, University of Minnesota Duluth, Duluth, MN 55812, USA and 2Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN 55455, USA Email: Brian W Kram - bkram@; Wayne W Xu - wxu@; Clay J Carter* - cjcarter@ * Corresponding author ?Equal contributors Published: 15 July 2009 Received: 7 April 2009 Accepted: 15 July 2009 BMC Plant Biology 2009, 9:92 doi:10.1186/1471-2229-9-92 This article is available from: /1471-2229/9/92 ? 2009 Kram et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Many flowering plants attract pollinators by offering a reward of floral nectar. Remarkably, the molecular events involved in the development of nectaries, the organs that produce nectar, as well as the synthesis and secretion of nectar itself, are poorly understood. Indeed, to date, no genes have been shown to directly affect the de novo production or quality of floral nectar. To address this gap in knowledge, the ATH1 Affymetrix? GeneChip array was used to systematically investigate the Arabidopsis nectary transcriptome to identify genes and pathways potentially involved in nectar production. Results: In this study, we identified a large number of genes differentially expressed between secretory lateral nectaries and non-secretory median nectary tissues, as well as between mature lateral nectaries (post-anthesis) and immature lateral nectaries (pre-anthesis). Expression withi