Uneven distribution of nucleoside transporters and intracellular enzymatic degradation prevent transport of intact [14C] adenosine across the sheep choroid plexus epithelium as a monolayer in primary culture 英文参考文献.docVIP

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Uneven distribution of nucleoside transporters and intracellular enzymatic degradation prevent transport of intact [14C] adenosine across the sheep choroid plexus epithelium as a monolayer in primary culture 英文参考文献.doc

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Uneven distribution of nucleoside transporters and intracellular enzymatic degradation prevent transport of intact [14C] adenosine across the sheep choroid plexus epithelium as a monolayer in primary culture 英文参考文献

Cerebrospinal Fluid Research BioMedCentral Research Open Access Uneven distribution of nucleoside transporters and intracellular enzymatic degradation prevent transport of intact [14C] adenosine across the sheep choroid plexus epithelium as a monolayer in primary culture Zoran B Redzic*1,4, Aleksandra J Isakovic2, Sonja T Misirlic Dencic2, Dusan Popadic3 and Malcolm B Segal1 Address: 1School of Biomedical Sciences, Kings College London, London, UK, 2Department of Biochemistry, School of Medicine, Belgrade, Serbia Montenegro, 3Department of Microbiology, School of Medicine, Belgrade, Serbia Montenegro and 4Department of Physiology, Faculty of Medicine, Kuwait, Kuwait Email: Zoran B Redzic* - redzic@.kw; Aleksandra J Isakovic - aleksandra-alisa@; Sonja T Misirlic Dencic - sonja@net.yu; Dusan Popadic - soul@eunet.yu; Malcolm B Segal - malcolm.segal@kcl.ac.uk * Corresponding author Published: 29 March 2006 Received: 05 September 2005 Accepted: 29 March 2006 Cerebrospinal Fluid Research2006, 3:4 doi:10.1186/1743-8454-3-4 This article is available from: /content/3/1/4 ? 2006Redzic et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Efflux transport of adenosine across the choroid plexus (CP) epithelium might contribute to the homeostasis of this neuromodulator in the extracellular fluids of the brain. The aim of this study was to explore adenosine transport across sheep CP epithelial cell monolayers in primary culture. Methods: To explore transport of adenosine across the CP epithelium, we have developed a method for primary culture of the sheep choroid plexus epithelial cells (CPEC) on plastic permeable supports and analysed [14C] adenosine transport across this cellular layer, [14C] a