颗粒裂解抗菌结构域在大肠杆菌中的重组表达.pdfVIP

颗粒裂解肽抗菌结构域在大肠杆菌中的重组表达 cationic antimicrobial peptide gene were separately inserted into the two MCS sites of the plasmid pACYCDuet-1. The recombiant plasmid was transformed into competent E.coli cells and co-expression was induced by IPTG. The results demonstrated that a higher expression yield was achieved by using the co-expression method. It will be helpful and meaningful for further study of antibacterial peptides and provides an economical but efficient preparation method. In order to improve the efficien