富含β-胡萝卜素转基因水稻EH基因水平转移研究.pdfVIP

上海农业学报 2016 ， 32(5) :16-21 Actα Agriculturae Shanghαi 001: 10. 15955/j. issnl创lO-3924. 2016. 05. 04 文章编号:1仪lO-3924( 却16 )05-016，，(滔 富含 p-胡萝卡素转基因水稻 EH 基因水平转移研究 吴洋洋l ，许燕l\ 胡瑞丽l\，任方方l ，赵凯2丸υ， 3 (υ1 上海师范大学生命与环境科学学院，上海 2∞2刀34;♂2 上海市农业科学院生物技术研究所，上海 2却0110侃6; 3 上海市农业遗传育种重点实验室，上海 201106;4 宁夏大学，银川 75∞21) 摘 要:以富含日-胡萝卡素转基因水稻 EH 喂养大鼠，在 90 d 喂养试验结束后，分别对大鼠十二指肠、回 肠、咬肌及肠道微生物进行相关基因 PCR 扩增，分析特异性片段在大鼠体内的转移情况。结果显示:试验中饲喂 含45% 转基因大米饲料、含45% 亲本‘中花 11 大米配合饲料和全价营养饲料的所有大鼠均未发生基因水平转 移，表明转基因水稻 EH 中的外源基因没有向大鼠的组织和肠道微生物进行转移。 关键词:转基因水稻;毒理性;基因水平转移;食用安全性评价 申圄分类号:Q78 文献标识码:A Studies 00 horizootal gene transfer with genetically modified rice EH rich in ß-carotene 1 1 1 1 WU Yang-yang ,XU Yan • , HU Rui-li , REN Fang-fang , 2 3 2 3 4 ZHAO Kai • .. ,TANG Xue-ming • .. ,ZHAO Xia0 (1 College 0/ Life and Environment Science ,Shanghαi Normal University ,Shanghαi 200234 ,Chinα; 2 Biotech Research Institute ,Shαnghai Academy 0/Agricultural Sciences ,Shanghαi 201106 ,China; 3 Shanghαi Key Laborαtory 0/Agricultural Genetics and Breeding ， Shanghαi 201106 , China; 4 Ningxia University , Yinchuan 750021 ， Chinα) Abstract: The rats were fed with transgenic rice EH enriched with beta carotene , after the 90-day feeding experiment , associated genes were amplified by PCR in duodenums , ileums ,masseter muscles and gut microbes of the rats respectively. Metastasis of specific fragments in rats was analyzed. 咀le results showed that horizontal gene transfer wasn t discovered in any rats in the experiment