不同激素处理对银条根生长发育的影响.docxVIP

银条茎尖的脱毒组织培养和植株再生 河南科技大学本科生毕业论文  PAGE II  PAGE IV 不同激素处理对银条根生长发育的影响 摘 要 银条（Stachys floridana Schuttl .ex Benth），又名地灵，系唇形科水苏属多年生蓄根草本植物，其地下根状茎圆状细长，肉质洁白，酷名银条似白银，故；据传是唐僧取经过程中所得，又被誉为“唐僧肉”。目前，全国人工种植面积很少，洛阳偃师县（市）是主要产地，约占全国产量的95%，仅春节左右大量上市，同时远销国内外，在市场上常供不应求[2]。本研究通过对银条根部分化的培养条件、不同处理对提高生根率的效果进行了研究，并利用不同外植体探讨了银条再生快繁的适宜途径。目的是获得脱毒种株并进性大量、快速的繁殖，以解决生产中银条由于结籽率低，且难以培养成苗，长期以根状茎进行无性繁殖所造成的病毒累积的问题，并为进一步进行银条的营养利用、生理代谢、生物育种研究奠定基础。 试验结果表明：银条的离体培养以MS作为基本培养基最为适宜，再生苗生长快速，植株健壮。茎段在MS+0.2mg/LNAA+活性炭3%的培养基中有最佳的生长状态，在较高的NAA或IBA浓度下，茎段下端愈伤样化严重，不定根产生量少，在IBA的作用下，银条茎叶纤弱不利于移栽成活。在MS+0.2mg/LNAA的培养基中，茎段生根量大，根型良好，茎叶粗壮，加入活性炭后，根的长度进一步增长，呈乳白色，移栽成活率达90%以上。 关 键 词：银条 茎段 不定根 植株再生 MS培养基 Stachys floridana Mreistem Culture and Plantlet Regeneration of Venom Tissue Removal ABSTRACT Stachys floridana, also known as Ling, Lamiaceae Stachys is a build root perennial herb, its underground rhizomes rounded slender, white meat, resembles silver, hence the name Stachys floridana; According to legend Journey to the West., also known as the official told us. The planted area rarely, Luoyang Yanshi county (city) is the main producer, accounting for about 95% of national output, a large number of listed only around the Spring Festival, and sold at home and abroad, often in short supply in the market . In this study, on Stachys floridana apical meristem culture conditions, different treatments to improve the effect of shoot tips regenerated plants detoxification rate and different explants suitable way to explore the Stachys floridana renewable rapid propagation. The results showed: Silver Cultured with MS as basic culture medium was the most suitable regeneration seedling growth, rapid, robust plants. Stems grow best in the culture medium MS+0.2mg/LNAA+ 3% of activated carbon, the higher NAA or IBA concentration, the lower end of serious stems callus, adventitious roots produced in small amounts, in the presence of IBA, silver leaf delicate is not conducive to the transplanting survival. In the culture medium wi