A Practical Guide to Rodent Islet Isolation and Assessment.docVIP

APracticalGuidetoRodentIsletIsolationandAssessment JeffreyD.Carter,StaceyB.Dula,KathrynL.Corbin,RunpeiWu, andCraigS.Nunemaker Abstract PancreaticisletsofLangerhanssecretehormonesthatarevitaltotheregulationofbloodglucoseandare, therefore, akeyfocus ofdiabetes research. Purifying viable andfunctional islets from the pancreas for studyisanintricate process. Thisreviewhighlights thekeyelements involved withmouseandratislet isolation,includingchoicesofcollagenase,thecollagenasedigestionprocess,purificationofisletsusing adensitygradient,andisletcultureconditions.Inaddition,thispaperreviewscommo