A Single-Step Purification and Molecular Characterization of Functional Shiga Toxin 2 Variants from Pathogenic Escherichia coli.docVIP

Toxins 2012, 4, 487-504; doi:10.3390/toxins4070487 OPEN ACCESS toxins ISSN 2072-6651 /journal/toxins Article A Single-Step Purification and Molecular Characterization of Functional Shiga Toxin 2 Variants from Pathogenic Escherichia coli Xiaohua He *, Beatriz Qui?ones, Stephanie McMahon and Robert E. Mandrell Western Regional Research Center, U.S. Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710, USA; E-Mails: beatriz.quinones@ (B.Q.); stephanie.mcmahon@ (S.M.); robert.mandrell@ (R.E.M.) * Author to whom correspondence should be addressed; E-Ma