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A systematic evaluation of whole genome amplification of bisulfite-modified DNA
Bundoetal.ClinicalEpigenetics2012,4:22
/content/4/1/22
METHODOLOGY
OpenAccess
Asystematicevaluationofwholegenome
amplificationofbisulfite-modifiedDNA
MikiBundo1,FumikoSunaga1,JunkoUeda2,KiyotoKasai3,TadafumiKato2andKazuyaIwamoto1*
Abstract
Background:StudyingDNAmethylationprofilesindetailshouldbethefirststepinepigeneticresearch.Although
sodiumbisulfitemodificationofgenomicDNAisthegoldstandardmethodforDNAmethylationanalysis,
thismethodresultsinthelossofthemajorityoftheDNAmaterial.Wholegenomeamplification(WGA)of
bisulfite-modifiedDNAisexpectedtoprovidearichsourceofmaterials,butitsvalidityhasno
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