Chromosome analysis of horse oocytes cultured in vitro.docVIP

cultured in vitro* 3 D 2 0YC, University of Guedph, Department of Biomedical Sciences; University of Guelph, Department of Clinical Studies; Guelph, Ontario, 3 Centre dins6mination (Received 23 October 1989; accepted 22 February 1990) Summary - Oocytes collected from slaughtered mares of unknown were cultured in modified Krebs Ringers bicarbonate supplemented with fetal calf serum (20%) and fixed for chromosome analysis. To determine the time required for nuclear maturation, oocytes were fixed either after 12 h (n = 21) 24 h (n = 21), 48 h (n = 20) without culture = 89% (n 30).