Combining SNP discovery from next-generation sequencing data with bulked segregant analysis (BSA) to fine-map genes in polyploid wheat.docVIP

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Combining SNP discovery from next-generation sequencing data with bulked segregant analysis (BSA) to fine-map genes in polyploid wheat.doc

Combining SNP discovery from next-generation sequencing data with bulked segregant analysis (BSA) to fine-map genes in polyploid wheat

Tricketal.BMCPlantBiology2012,12:14 /1471-2229/12/14 METHODOLOGY ARTICLE OpenAccess CombiningSNPdiscoveryfromnext-generation sequencingdatawithbulkedsegregantanalysis (BSA)tofine-mapgenesinpolyploidwheat MartinTrick1,NikolaiMariaAdamski1,SarahGMugford1,Cong-CongJiang1,MelanieFebrer2and CristobalUauy1,3* Abstract Background:Nextgenerationsequencing(NGS)technologiesareprovidingnewwaystoacceleratefine-mapping andgeneisolationinmanyspecies.Todate,themajorityoftheseeffortshavefocusedondiploidorganismswith readilyavailablewholegenomesequenceinformation.Inthisstudy,asaproofofconcept,wetestedtheuse

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