Combining SNP discovery from next-generation sequencing data with bulked segregant analysis (BSA) to fine-map genes in polyploid wheat.docVIP
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Combining SNP discovery from next-generation sequencing data with bulked segregant analysis (BSA) to fine-map genes in polyploid wheat
Tricketal.BMCPlantBiology2012,12:14
/1471-2229/12/14
METHODOLOGY ARTICLE
OpenAccess
CombiningSNPdiscoveryfromnext-generation
sequencingdatawithbulkedsegregantanalysis
(BSA)tofine-mapgenesinpolyploidwheat
MartinTrick1,NikolaiMariaAdamski1,SarahGMugford1,Cong-CongJiang1,MelanieFebrer2and
CristobalUauy1,3*
Abstract
Background:Nextgenerationsequencing(NGS)technologiesareprovidingnewwaystoacceleratefine-mapping
andgeneisolationinmanyspecies.Todate,themajorityoftheseeffortshavefocusedondiploidorganismswith
readilyavailablewholegenomesequenceinformation.Inthisstudy,asaproofofconcept,wetestedtheuse
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