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Effects of dexamethasone on TNF-alpha-induced release of cytokines from purified human blood eosinophils.doc

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Clinical and Molecular Allergy BioMedCentral Research Open Access Effects of dexamethasone on TNF-alpha-induced release of cytokines from purified human blood eosinophils Iain Uings?1, Ilaria Puxeddu?2, Vladislav Temkin2, Susan J Smith2, Dilniya Fattah1, Keith P Ray1 and Francesca Levi-Schaffer*2 Address: 1Cell Biology Unit, Glaxo Wellcome SKB, Gunnels Wood Stevenage, Herts, SG1 2NY, UK and 2Department of Pharmacology, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel Email: Iain Uings - iju44964@; Ilaria Puxeddu - ilarip@yahoo.it; Vladislav Temkin - v-temkin@; Susan J Smith - susan.smith@imperial.ac.uk; Dilniya Fattah - dilniya.7.fattah@; Keith P Ray - kpr0136@; Francesca Levi- Schaffer* - fls@cc.huji.ac.il * Corresponding author ?Equal contributors Published: 27 April 2005 Received: 11 January 2005 Accepted: 27 April 2005 Clinical and Molecular Allergy 2005, 3:5 doi:10.1186/1476-7961-3-5 This article is available from: /content/3/1/5 ? 2005 Uings et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. TNF-αeosinophilsdexamethasoneallergic inflammationcytokine Summary Background: TNF-alpha is an important mediator in allergy also for its effects on eosinophils. Methods: The effect of dexamethasone on TNF-alpha induced eosinophils survival, degranulation (ECP), cytokines release (IL-8, GM-CSF) and adhesion to VCAM-1, ICAM-1 and IgG coated wells (EPO release) were evaluated. Results: The drug inhibited IL-8 and GM-CSF production, but not viability, degranulation or adhesion in human peripheral blood eosinophils. Conclusion: These results indicate that part of the activity of glucocorticosteroids on eosinophils may be mediated by their ability to inhibit cytokine secretion that