Effects of hyaluronan treatment on lipopolysaccharide-challenged fibroblast-like synovial cells.docVIP

Available online /content/9/1/R1 Research article Open Access Vol 9 No 1 Effects of hyaluronan treatment on lipopolysaccharide-challenged fibroblast-like synovial cells Kelly S Santangelo1, Amanda L Johnson1, Amy S Ruppert2 and Alicia L Bertone1 1Department of Clinical Sciences, College of Veterinary Medicine, The Ohio State University, 1900 Coffey Road, Columbus OH 43210, USA 2Center for Biostatistics, The Ohio State University, 320 West 10th Avenue, Columbus OH 43210, USA Corresponding author: Alicia L Bertone, bertone.1@ Received: 3 Jun 2006 Revisions requested: 20 Jul 2006 Revisions received: 19 Dec 2006 Accepted: 10 Jan 2007 Published: 10 Jan 2007 Arthritis Research Therapy 2007, 9:R1 (doi:10.1186/ar2104) This article is online at: /content/9/1/R1 ? 2007 Santangelo et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Numerous investigations have reported the efficacy of increased IL-6 production 15-fold (P 0.05), increased MMP3 production threefold (P 0.05), and generated a profile of gene expression changes typical of LPS (P 0.005). Importantly, LPS exposure at this concentration did not alter the cell viability. Higher molecular weight HA decreased the morphologic change (P 0.05) associated with LPS exposure. Both lower and higher molecular weight HA significantly altered a similar set of 21 probe sets (P 0.005), which represented decreased exogenous hyaluronan (HA) in modulating acute and chronic inflammation. The current study was performed to determine the in vitro effects of lower and higher molecular weight HA on (LPS)-challenged fibroblast-like synovial lipopolysaccharide cells. Normal synovial fibroblasts were cultured in triplicate to one of four groups: grou