Effects of rehydration nutrients on H2S metabolism and formation of volatile sulfur compounds by the wine yeast VL3.docVIP
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Effects of rehydration nutrients on H2S metabolism and formation of volatile sulfur compounds by the wine yeast VL3
Winteretal.AMBExpress2011,1:36
/content/1/1/36
ORIGINAL
OpenAccess
EffectsofrehydrationnutrientsonH2S
metabolismandformationofvolatilesulfur
compoundsbythewineyeastVL3
GalWinter1,2,PaulAHenschke2,VincentJHiggins1,3,MaurizioUgliano2,4 andChrisDCurtin2*
Abstract
Inwinemaking,nutrientsupplementationisacommonpracticeforoptimisingfermentationandproducingquality
wine.Nutritionallysuboptimalgrapejuicesareoftenenrichedwithnutrientsinordertomanipulatetheproduction
ofyeastaromacompounds.Nutrientsarealsoaddedtoactivedryyeast(ADY)rehydrationmediatoenhance
subsequentfermentationperformance.Inthisstudywedemonstratethatnutrientsupplementationatrehydration
alsohasasignificanteffectontheformationofvolatilesulfurcompoundsduringwinefermentations.The
concentrationofthe‘fruity’aromacompounds,thepolyfunctionalthiols3-mercaptohexan-1-ol(3MH)and3-
mercaptohexylacetate(3MHA),wasincreasedwhiletheconcentrationofthe‘rottenegg’aromacompound,
hydrogensulfide(H2S),wasdecreased.Nutrientsupplementationoftherehydrationmediaalsochangedthe
kineticsofH2SproductionduringfermentationbyadvancingonsetofH2Sproduction.Microarrayanalysisrevealed
thatthiswasnotduetoexpressionchangeswithinthesulfateassimilationpathway,whichisknowntobeamajor
contributortoH2Sproduction.Togaininsightintopossiblemechanismsresponsibleforthiseffect,acomponent
oftherehydrationnutrientmix,thetri-peptideglutathione(GSH)wasaddedatrehydrationandstudiedforits
subsequenteffectsonH2Sformation.GSHwasfoundtobetakenupduringrehydrationandtoactasasourcefor
H2Sduringthefollowingfermentation.Thesefindingsrepresentapotentialapproachformanagingsulfuraroma
productionthroughtheuseofrehydrationnutrients.
Keywords:Rehydration,yeast,nutrients,H2S,hydrogen-sulfide,GSH,glutathione
Introduction
inorganicnitrogen,usuallyintheformofdiammonium
Inmanyviticulturalregionsthenaturalnutrientcompo- phosphate (DAP) and organic nutrient preparations.
sition of grape juice is considered suboptimal and may Thelatter aretypically prepared frominactive orauto-
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