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Embryonic Stem Cells Maintain an Undifferentiated State on Dendrimer-Immobilized Surface with d-Glucose Display.doc

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Embryonic Stem Cells Maintain an Undifferentiated State on Dendrimer-Immobilized Surface with d-Glucose Display

Polymers 2011, 3, 2078-2087; doi:10.3390/polym3042078 OPEN ACCESS polymers ISSN 2073-4360 /journal/polymers Communication Embryonic Stem Cells Maintain an Undifferentiated State on Dendrimer-Immobilized Surface with D-Glucose Display Shohreh Mashayekhan 1,*, Mee-Hae Kim 2, Masahiro Kino-oka 2, Jun-ichi Miyazaki 3 and Masahito Taya 4 1 Department of Chemical Petroleum Engineering, Sharif University of Technology, Azadi Ave, Tehran, 11365-8639, Iran 2 Laboratory of BioProcess Systems Engineering, Department of Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan; E-Mails: mh-kim@bio.eng.osaka-u.ac.jp (M.-H.K.); kino-oka@bio.eng.osaka-u.ac.jp (M.K.-O.) 3 Division of Stem Cell Regulation Research (G6), Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan; E-Mail: jimiyaza@nutri.med.osaka-u.ac.jp (J.I.M.) 4 Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka, 560-8531, Japan; E-Mail: taya@cheng.es.osaka-u.ac.jp (M.T.) * Author to whom correspondence should be addressed; E-Mail: mashayekhan@; Tel./Fax: +98-21-661-664-14. Received: 2 November 2011; in revised form: 28 November 2011 / Accepted: 2 December 2011 / Published: 5 December 2011 Abstract: In serial passaging cultures of mouse embryonic stem (ES) cells, we employed a dendrimer-immobilized substrate that displayed D-glucose as a terminal ligand. The D-glucose-displaying dendrimer (GLU/D) surface caused the ES cells to form loosely attached spherical colonies, while those on a gelatin-coated surface formed flatter colonies that were firmly attached to the surface. Despite the morphological similarities between the colonies on the GLU/D surface and aggregates on a conventional bacteriological dish, immunostaining and RT-PCR analyses revealed the maintenance of cells within the spherical colonies on the GLU/D surface in an undiffer